Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473]
- RabMAb
- Recombinant
- Advanced Validation
- 20ul selling size
- 각 태그의 뜻
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(7 제품이 사용된 논문 )
Rabbit Recombinant Monoclonal KAT7 / Hbo1 / MYST2 antibody. Suitable for ChIC/CUT&RUN-seq, IP, WB, ICC/IF, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 7 publications.
대체 명칭 보기
HBO1, HBOa, MYST2, KAT7, Histone acetyltransferase KAT7, Histone acetyltransferase binding to ORC1, Lysine acetyltransferase 7, MYST-2
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (Human epithelial cells from embryonic kidney) cells labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on HEK-293 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab190908 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/500 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/500 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human testis is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IP
Supplier Data
Immunoprecipitation - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
KAT7 / Hbo1 / MYST2 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab190908 at 1/60 dilution. Western blot was performed from the immunoprecipitate using ab190908 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1 : Jurkat whole cell lysate 10ug (Input).
Lane 2 : ab190908 IP in Jurkat whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab190908 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds.
All lanes:
Immunoprecipitation - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908)
Predicted band size: 71 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on mouse colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling KAT7 / Hbo1 / MYST2 with ab190908 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on rat testis is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908) at 1/1000 dilution
All lanes:
human breast cancer lysate at 40 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution
Predicted band size: 71 kDa
Observed band size: 75 kDa
false
Exposure time: 3min
- WB
Supplier Data
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908) at 1/5000 dilution
Lane 1:
HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 10 µg
Lane 2:
MCF7 (Human breast adenocarcinoma cell line) cell lysate at 10 µg
Lane 3:
NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 71 kDa
Observed band size: 75 kDa
false
Exposure time: 3min
- ChIC/CUT&RUN-seq
Lab
ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab190908 [EPR18473]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
- ChIC/CUT&RUN-seq
Lab
ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab190908 [EPR18473]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
- ChIC/CUT&RUN-seq
Lab
ChIC/CUT&RUN sequencing - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 HeLa (human cervix adenocarcinoma epithelial cell) cells and 5 µg of ab190908 [EPR18473]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
- WB
Supplier Data
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (AB190908)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] (ab190908) at 1/5000 dilution
Lane 1:
C6 (Rat glial tumor cells) cell lysate at 10 µg
Lane 2:
RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 71 kDa
Observed band size: 75 kDa
false
Exposure time: 30s
관련 conjugated 항체와 다양한 조성의 항체 (1)
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Anti-KAT7 / Hbo1 / MYST2 antibody [EPR18473] - BSA and Azide free
Reactivity 정보
제품 세부 정보
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein plays a significant role in transcription regulation and DNA replication. KAT7 is a component of the HBO1 complex working together with other proteins like JADE1/2/3 BRPF1/2/3 and ING4/5 making it integral to cell cycle progression and transcriptional activation. This complex is essential for initiating DNA replication as it acetylates histones and regulates chromatin accessibility necessary for DNA synthesis.
Pathways
KAT7/Hbo1/MYST2 is actively engaged in the transcriptional regulatory network and DNA replication pathways. It influences gene expression through its interaction with the transcriptional machinery and by modifying the chromatin structure. Within these pathways it interacts with proteins like BRPF1 and ING5 enhancing transcription and replication. This positions KAT7 as an important component in ensuring cellular growth and division through its histone acetylation activities.
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타겟 정보
제품이 사용된 논문 (7)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Nature structural & molecular biology 32:1255-1267 PubMed40186025
2025
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The EMBO journal 44:1322-1353 PubMed39815006
2025
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BMC cancer 23:586 PubMed37365518
2023
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Biomedical research (Tokyo, Japan) 44:105-115 PubMed37258203
2023
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Cell death discovery 6:89 PubMed33014433
2020
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International journal of biological sciences 16:2340-2356 PubMed32760202
2020
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International journal of chronic obstructive pulmo 15:15-24 PubMed32021140
2020
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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