AB94578

Anti-KCNT1/SLACK antibody [N3/26]

Name: Anti-KCNT1/SLACK antibody [N3/26] (ab94578) | Abcam
Brand: Abcam Limited
SKU: AB94578
Rating: 5 (1 reviews)

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(4 제품이 사용된 논문 )

Anti-KCNT1/SLACK antibody [N3/26] (ab94578) is a mouse monoclonal antibody detecting KCNT1/SLACK in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.

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3 이미지

Supplier Data

Western blot - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Membrane was blocked with 1.5% BSA for 30 minutes at room temperature. Primari antibody was incubated for 2 hours at room temperature.

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Western blot - Anti-KCNT1/SLACK antibody [N3/26] (ab94578) at 1/1000 dilution

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Rat brain lysate at 15 µg

Secondary

All lanes:

HRP-conjugated sheet anti-mouse IgG

Predicted band size: 138 kDa

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Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Flow Cyt

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Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Overlay histogram showing SH-SY5Y cells stained with ab94578 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94578, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse backskin staining KCNT1/SLACK with ab94578 at 1 : 100 for 1 hour at RT. Secondary Antibody : FITC Goat Anti-Mouse (green) at 1 : 50 for 1 hour at RT.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

N3/26

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Rat Kcnt1 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Z258

Specificity

Detects ~140kDa. Weak human detection. Does not cross-react with KCNT2/Slo2.1/Slick.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-KCNT1/SLACK antibody [N3/26] (ab94578) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of KCNT1/SLACK?
Anti-KCNT1/SLACK [N3/26] (ab94578) specifically detects a band for KCNT1/SLACK (UniProt: Q9Z258) at a molecular weight of 137kDa.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Sodium-activated K(+) channel (PubMed : 37494189). Acts as an important mediator of neuronal membrane excitability (PubMed : 37494189). Contributes to the delayed outward currents (By similarity). Regulates neuronal bursting in sensory neurons (By similarity). Contributes to synaptic development and plasticity (By similarity).

See full target information KCNT1

대체 명칭 보기

KIAA1422, SLACK, Slo2.2, KCNT1, Potassium channel subfamily T member 1, KCa4.1, KNa1.1, Sodium and chloride-activated ATP-sensitive potassium channel Slo2.2

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

eLife 12: PubMed37162194

2023

Tiered sympathetic control of cardiac function revealed by viral tracing and single cell transcriptome profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Sachin Sharma,Russell Littman,John D Tompkins,Douglas Arneson,Jaime Contreras,Al-Hassan Dajani,Kaitlyn Ang,Amit Tsanhani,Xin Sun,Patrick Y Jay,Herbert Herzog,Xia Yang,Olujimi A Ajijola

Neuroscience bulletin 37:669-683 PubMed33779892

2021

A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yize Li,Linlin Zhang,Jing Li,Chunyan Wang,Yi Chen,Yuan Yuan,Keliang Xie,Guolin Wang,Yonghao Yu

Cell reports 33:108303 PubMed33113364

2020

Reduced GABAergic Neuron Excitability, Altered Synaptic Connectivity, and Seizures in a KCNT1 Gain-of-Function Mouse Model of Childhood Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Amy N Shore,Sophie Colombo,William F Tobin,Sabrina Petri,Erin R Cullen,Soledad Dominguez,Christopher D Bostick,Michael A Beaumont,Damian Williams,Dion Khodagholy,Mu Yang,Cathleen M Lutz,Yueqing Peng,Jennifer N Gelinas,David B Goldstein,Michael J Boland,Wayne N Frankel,Matthew C Weston

Neurobiology of learning and memory 155:173-179 PubMed30053575

2018

Neocortical SHANK1 regulation of forebrain dependent associative learning.

Applications

Unspecified application

Species

Unspecified reactive species

Sean M Collins,Roberto Galvez

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KCNT1/SLACK antibody [N3/26]

Western blot - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Flow Cytometry - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [N3/26] (AB94578)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise