AB308193

Anti-KDM6A antibody [HL1731]

Recombinant
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Rabbit Recombinant Monoclonal KDM6A / UTX antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Cat, Dog samples. Immunogen corresponding to Recombinant Fragment Protein within Human KDM6A.

대체 명칭 보기

UTX, KDM6A, Lysine-specific demethylase 6A, Histone demethylase UTX, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein, [histone H3]-trimethyl-L-lysine(27) demethylase 6A

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Immunocytochemistry/ Immunofluorescence - Anti-KDM6A antibody [HL1731] (AB308193)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KDM6A antibody [HL1731] (AB308193)

For immunofluorescence analysis, MCF-7 cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of KDM6A protein using ab308193 at 1/500 dilution (green).

Nuclei (blue) is stained with fluoroshield with DAPI

Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

Immunohistochemistry paraffin embedded sections - Anti-KDM6A antibody [HL1731] (AB308193)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-KDM6A antibody [HL1731] (AB308193)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling KDM6A protein with ab308193 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM6A antibody [HL1731] (AB308193)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM6A antibody [HL1731] (AB308193)

Immunohistochemical analysis of paraffin-embedded Cat intestine/colon tissue labelling KDM6A with ab308193 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM6A antibody [HL1731] (AB308193)

Immunohistochemical analysis of paraffin-embedded dog brain tissue labelling KDM6A with ab308193 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1731

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Dog, Cat

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KDM6A. The exact immunogen used to generate this antibody is proprietary information.

O15550

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Cat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Dog": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code (PubMed : 17713478, PubMed : 17761849, PubMed : 17851529). Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27' (PubMed : 17713478, PubMed : 17761849, PubMed : 17851529). Plays a central role in regulation of posterior development, by regulating HOX gene expression (PubMed : 17851529). Demethylation of 'Lys-27' of histone H3 is concomitant with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A (PubMed : 17761849). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (By similarity).

See full target information KDM6A

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KDM6A antibody [HL1731]

Immunocytochemistry/ Immunofluorescence - Anti-KDM6A antibody [HL1731] (AB308193)

Immunohistochemistry paraffin embedded sections - Anti-KDM6A antibody [HL1731] (AB308193)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM6A antibody [HL1731] (AB308193)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM6A antibody [HL1731] (AB308193)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

Product promise