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AB237863

Anti-Ki67 antibody [MKI67/2462] - BSA and Azide free

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Mouse Monoclonal Ki67 antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MKI67 aa 2250 to C-terminus.

대체 명칭 보기

Proliferation marker protein Ki-67, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67, Antigen Ki67, MKI67

4 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] - BSA and Azide free (AB237863)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] - BSA and Azide free (AB237863)

Formalin-fixed, paraffin-embedded human tonsil-skin tissue stained for Ki67 using ab237863 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] - BSA and Azide free (AB237863)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] - BSA and Azide free (AB237863)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab237863 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] - BSA and Azide free (AB237863)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [MKI67/2462] - BSA and Azide free (AB237863)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab237863 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-Ki67 antibody [MKI67/2462] - BSA and Azide free (AB237863)
  • Protein Array

Supplier Data

Protein Array - Anti-Ki67 antibody [MKI67/2462] - BSA and Azide free (AB237863)

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab237863.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

MKI67/2462

Isotype

IgG2b

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Protein Array

applications

Immunogen

Recombinant Fragment Protein within Human MKI67 aa 2250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P46013

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at RT</p><p>Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.</p>" }, "Recombinant full length protein - Human": { "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
Purified from Bioreactor Concentrate by Protein A/G.
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ki67 is a nuclear protein often referred to as MKI67 with a molecular weight of approximately 345 kDa. This protein is strongly associated with cell proliferation. Scientists commonly use Ki67 staining techniques to identify and quantify proliferating cells in tissues. Ki67 is expressed in the nucleus during active cell cycle phases—G1 S G2 and M—but not in resting cells in G0 phase. It is detectable through methods such as Ki67 immunofluorescence and Ki67 flow cytometry which are important for analyzing cell division.
Biological function summary

The Ki67 protein plays an essential role in cellular proliferation processes. It does not form part of a stable complex but interacts transiently with other cell cycle-related proteins. Research indicates that Ki67 maintains the structure of the perichromosomal layer during mitosis impacting chromosome separation. It is particularly active in tissues with high cell turnover such as bone marrow and lymphoid organs.

Pathways

Ki67 influences several key cellular pathways that control cell proliferation and differentiation. The protein acts within the regulation of the cell cycle and the PI3K/AKT signaling pathway important for cell growth and survival. Within these pathways Ki67 interacts with proteins like cyclin-dependent kinases (CDKs) which regulate transitions between different phases of the cell cycle.

Ki67 has significant implications in oncology and can be used as a biomarker for cancer prognosis. Its expression levels help determine the aggressiveness of tumors especially in breast cancer and prostate cancer. High Ki67 levels correlate with poor prognosis as it indicates rapid cell division. In cancer Ki67 associates with p53 a protein that regulates the cell cycle and function as a tumor suppressor.

제품 프로토콜

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타겟 정보

Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis (PubMed : 27362226, PubMed : 32879492, PubMed : 35513709, PubMed : 39153474). Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed : 27362226). During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface (PubMed : 27362226). The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier : the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed : 27362226). During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space (PubMed : 32879492, PubMed : 39153474). Mechanistically, dephosphorylation during mitotic exit and simultaneous exposure of a conserved basic patch induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface, promoting coalescence of neighboring chromosome surfaces and clustering of chromosomes (PubMed : 39153474). Binds premature ribosomal RNAs during anaphase; promoting liquid-liquid phase separation (PubMed : 28935370, PubMed : 39153474). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed : 10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization; it is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in mitotic chromosome (PubMed : 24867636).
See full target information MKI67

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS pathogens 20:e1012768 PubMed39652618

2024

Fatal Lassa fever in cynomolgus monkeys is associated with systemic viral dissemination and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jimmy Hortion,Emeline Perthame,Blaise Lafoux,Laura Soyer,Stéphanie Reynard,Alexandra Journeaux,Clara Germain,Hélène Lopez-Maestre,Natalia Pietrosemoli,Nicolas Baillet,Séverine Croze,Catherine Rey,Catherine Legras-Lachuer,Sylvain Baize
제품이 사용된 논문 모두 보기

Product promise

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