AB226255

Anti-KIAA0101 antibody

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(6 제품이 사용된 논문 )

Rabbit Polyclonal KIAA0101 antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human PCLAF aa 1-50.

대체 명칭 보기

KIAA0101, NS5ATP9, PAF, L5, PCLAF, PCNA-associated factor, Hepatitis C virus NS5A-transactivated protein 9, Overexpressed in anaplastic thyroid carcinoma 1, PCNA-associated factor of 15 kDa, PCNA-clamp-associated factor, HCV NS5A-transactivated protein 9, OEATC-1, PAF15, p15PAF

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0101 antibody (AB226255)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0101 antibody (AB226255)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for KIAA0101 using ab226255 at 1/1000 dilution in immunohistochemical analysis.

Detection : DAB staining. Counterstain : Hematoxylin (blue).

Supplier Data

Immunoprecipitation - Anti-KIAA0101 antibody (AB226255)

KIAA0101 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using RIPA buffer; 1 mg for IP, 20% of IP loaded) with ab226255 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226255 at 1 μg/ml.

Lane 1 : ab226255 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-KIAA0101 antibody (ab226255)

Predicted band size: 12 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human PCLAF aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q15004

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab226255 was affinity purified using an epitope specific to PAF immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number.

See full target information PCLAF

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Thoracic cancer 13:1751-1762 PubMed35524161

2022

Circ_0005231 promotes the progression of esophageal squamous cell carcinoma via sponging miR-383-5p and regulating KIAA0101.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiguang Sun,Shaowei Zhang,Nan Zhang,Jianxin Wang,Jindong Wang,Junfeng Liu

Oncology reports 46: PubMed34296306

2021

Circular RNA TADA2A promotes proliferation and migration via modulating of miR‑638/KIAA0101 signal in non‑small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Zhang,Hongmin Yao,Ying Li,Lu Yang,Liang Zhang,Jinxin Chen,Yong Wang,Xia Li

Experimental and therapeutic medicine 22:969 PubMed34335911

2021

Curcumin inhibits prostate cancer progression by regulating the miR-30a-5p/PCLAF axis.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Pan,Jian Sha,Wenyao Lin,Yuxiong Wang,Tingzhang Bian,Jianming Guo

Cell cycle (Georgetown, Tex.) 20:1010-1020 PubMed33970778

2021

PAF enhances cancer stem cell properties via β-catenin signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Li,Xu-Dong Mu,Juan-Rong Song,Peng-Tao Zhai,Yuan Cheng,Yao Le,Zhu-Bin Li

Molecular cell 81:1698-1714.e6 PubMed33626321

2021

PAF remodels the DREAM complex to bypass cell quiescence and promote lung tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Moon Jong Kim,Christopher Cervantes,Youn-Sang Jung,Xiaoshan Zhang,Jie Zhang,Sung Ho Lee,Sohee Jun,Larisa Litovchick,Wenqi Wang,Junjie Chen,Bingliang Fang,Jae-Il Park

Biochemical and biophysical research communication 503:600-606 PubMed29902451

2018

KIAA0101 inhibition suppresses cell proliferation and cell cycle progression by promoting the interaction between p53 and Sp1 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lv,Benhua Su,Yuyang Li,Chong Geng,Na Chen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KIAA0101 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0101 antibody (AB226255)

Immunoprecipitation - Anti-KIAA0101 antibody (AB226255)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (6)

Product promise