JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB5629

Anti-KIF5B antibody

5

(2 리뷰들)

|

(20 제품이 사용된 논문 )

Rabbit Polyclonal KIF5B antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human KIF5B aa 350-400.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (AB5629)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KIF5B antibody (AB5629)

ab5629 labelling KIF5B (green) in HeLa cells. Cells fixed in 3.2% paraformaldehyde and permeabilized with 0.2% Triton X-100 for 30 minutes at 37°C were blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 250) overnight at 4°C. A fluorescently-conjugated Goat anti-rabbit IgG was used as the secondary antibody (1 : 2000). Images were taken on wide-field fluorescent microscope at 63X magnification.

Western blot - Anti-KIF5B antibody (AB5629)
  • WB

Supplier Data

Western blot - Anti-KIF5B antibody (AB5629)

All lanes:

Western blot - Anti-KIF5B antibody (ab5629) at 1/4000 dilution

Lane 1:

Mouse Cerebellum whole cell extract at 30 µg

Lane 2:

Rat Brain whole cell extract at 30 µg

Lane 3:

Mouse Brain whole cell extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution

Predicted band size: 110 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human KIF5B aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

P33176

Specificity

This antibody is specific for KIF5B and does not detect other kinesin isotypes.

style
left-column

대체품

Primary Antibodies

AB167429

Anti-KIF5B+KIF5C antibody [EPR10276(B)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-KIF5B+KIF5C antibody [EPR10276(B)] (AB167429)

  • 4
  • 3
Primary Antibodies

AB25715

Anti-KIF5B antibody

Western blot - Anti-KIF5B antibody (AB25715)

  • 4
  • 2
Primary Antibodies

AB237169

PE Anti-KIF5B+KIF5C antibody [EPR10276(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-KIF5B+KIF5C antibody [EPR10276(B)] (AB237169)

  • 0
  • 0
Primary Antibodies

AB232350

Anti-KIF5B+KIF5C antibody [EPR10276(B)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-KIF5B+KIF5C antibody [EPR10276(B)] - BSA and Azide free (AB232350)

  • 0
  • 0
Primary Antibodies

AB151558

Anti-KIF5B antibody [EPR10277(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5B antibody [EPR10277(B)] (AB151558)

  • 5
  • 1

보완 제품

Primary Antibodies

AB5628

Anti-KIF5A antibody

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (AB5628)

  • 5
  • 3
Primary Antibodies

AB193352

Anti-KIF5C antibody [EPR16224-32]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-KIF5C antibody [EPR16224-32] (AB193352)

  • 0
  • 0
Primary Antibodies

AB108629

Anti-TUBA1B antibody [EPR1333] - Loading Control

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Flow Cytometry (Intracellular) - Anti-TUBA1B antibody [EPR1333] - Loading Control (AB108629)

  • 0
  • 0
Primary Antibodies

AB22595

Anti-Calnexin antibody - ER Marker

Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (AB22595)

  • 5
  • 28
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/6000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/6000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed : 20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity). Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends (Probable). Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (PubMed : 24088571).
See full target information KIF5B

대체 명칭 보기

KNS, KNS1, KIF5B, Kinesin-1 heavy chain, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, UKHC
style
left-column

제품이 사용된 논문 (20)

Recent publications for all applications. Explore the 전체 목록 and refine your search

NPJ Parkinson's disease 11:114 PubMed40328804

2025

Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoman Yang,Zhuoran Ma,Piaopiao Lian,Yi Wu,Ke Liu,Zhaoyuan Zhang,Zhicheng Tang,Yan Xu,Xuebing Cao

Nature communications 15:10829 PubMed40016183

2025

Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Hei Li,Noortje Kersten,Nazmiye Özkan,Dan T M Nguyen,Max Koppers,Harm Post,Maarten Altelaar,Ginny G Farias

The Journal of cell biology 222: PubMed37213076

2023

Inhibition of endolysosome fusion increases exosome secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Ganesh Vilas Shelke,Chad D Williamson,Michal Jarnik,Juan S Bonifacino

eLife 12: PubMed36897066

2023

Rab10 regulates the sorting of internalised TrkB for retrograde axonal transport.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Marcelo Lazo,Giampietro Schiavo

BMC molecular and cell biology 23:26 PubMed35794526

2022

Effects of the media conditioned by various macrophage subtypes derived from THP-1 cells on tunneling nanotube formation in pancreatic cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Wei Lee,Chia-Chen Kuo,Chi-Jung Liang,Huei-Jyuan Pan,Chia-Ning Shen,Chau-Hwang Lee

Nature 599:662-666 PubMed34789877

2021

Herpesviruses assimilate kinesin to produce motorized viral particles.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin E Pegg,Sofia V Zaichick,Ewa Bomba-Warczak,Vladimir Jovasevic,DongHo Kim,Himanshu Kharkwal,Duncan W Wilson,Derek Walsh,Patricia J Sollars,Gary E Pickard,Jeffrey N Savas,Gregory A Smith

International journal of molecular sciences 22: PubMed33466445

2021

Intermittent Hypoxic Conditioning Rescues Cognition and Mitochondrial Bioenergetic Profile in the Triple Transgenic Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sónia C Correia,Nuno J Machado,Marco G Alves,Pedro F Oliveira,Paula I Moreira

Journal of cell science 133: PubMed32661088

2020

Microtubule motor transport in the delivery of melanosomes to the actin-rich apical domain of the retinal pigment epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Jiang,Antonio E Paniagua,Stefanie Volland,Hongxing Wang,Adarsh Balaji,David G Li,Vanda S Lopes,Barry L Burgess,David S Williams

The Journal of cell biology 219: PubMed31910261

2020

Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain.

Applications

Unspecified application

Species

Unspecified reactive species

Min Lee,Yen-Chen Liu,Chen Chen,Chi-Huan Lu,Shao-Tzu Lu,Tzyy-Nan Huang,Meng-Tsung Hsu,Yi-Ping Hsueh,Pei-Lin Cheng

Laboratory investigation; a journal of technical m 99:1822-1834 PubMed31409893

2019

Lipid droplet velocity is a microenvironmental sensor of aggressive tumors regulated by V-ATPase and PEDF.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Nardi,Philip Fitchev,Kyrsten M Brooks,Omar E Franco,Kevin Cheng,Simon W Hayward,Michael A Welte,Susan E Crawford
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com