AB241996

Anti-Kir6.1/KCNJ8 antibody [S366-60]

Name: Anti-Kir6.1/KCNJ8 antibody [S366-60]
Brand: Abcam Limited
SKU: AB241996
Rating: 5 (1 reviews)

(1 리뷰)

(6 제품이 사용된 논문 )

Mouse Monoclonal Kir6.1/KCNJ8 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat ATP-sensitive inward rectifier potassium channel 8 aa 300 to C-terminus.

대체 명칭 보기

ATP-sensitive inward rectifier potassium channel 8, Inward rectifier K(+) channel Kir6.1, uKATP-1, KCNJ8

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Kir6.1/KCNJ8 antibody [S366-60] (AB241996)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Kir6.1/KCNJ8 antibody [S366-60] (AB241996)

Immunocytochemistry/Immunofluorescence analysis of Neuroblastoma cells (SH-SY5Y) using Anti-Kir6.1/KCNJ8 antibody [S366-60] (ab241996). Fixation : 4% PFA for 15 min. Primary Antibody : ab241996 at 1 : 100 for overnight at 4°C with slow rocking. Secondary Antibody : AlexaFluor 488 at 1 : 1000 for 1 hour at RT. Counterstain : Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1 : 800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Anti-Kir6.1/KCNJ8 antibody [S366-60] (ab241996) (D) Merged.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Kir6.1/KCNJ8 antibody [S366-60] (AB241996)

4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for Kir6.1/KCNJ8 (green, C) using ab241996 at 1/100 dilution for 60 minutes at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a compsite image.

Supplier Data

Western blot - Anti-Kir6.1/KCNJ8 antibody [S366-60] (AB241996)

Primary incubation for 16 hrs at 4°C.

Secondary incubation for 1 hr at RT.

Blocked for 1 hr at RT.

All lanes:

Western blot - Anti-Kir6.1/KCNJ8 antibody [S366-60] (ab241996) at 1/1000 dilution

All lanes:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/200 dilution

Predicted band size: 47 kDa

Observed band size: 45 kDa

true

Exposure time: 6min

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

S366-60

Isotype

IgG2a

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Rat ATP-sensitive inward rectifier potassium channel 8 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q63664

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Kir6.1 also known as KCNJ8 is an integral part of a potassium channel complex. It forms the inwardly rectifying potassium channel (Kir) family that allows potassium ions to flow into cells. The Kir6.1 channel often a part of the larger ATP-sensitive potassium channels (K_ATP channels) regulates cellular membrane potentials. The protein mass of Kir6.1 is approximately 45 kDa. You can find Kir6.1 expressed in various tissues including the heart skeletal muscle and smooth muscle as well as in pancreatic beta cells.

Biological function summary

Kir6.1 plays a significant role in linking cellular energy status to membrane excitability through the modulation of potassium ion conductance. It forms a complex with SUR2 (sulfonylurea receptor 2) subunits collectively constituting the K_ATP channel complex. This channel complex senses changes in intracellular ATP levels and its activity influences insulin secretion in the pancreas and vascular tone in smooth muscle. By coupling cellular metabolic status to the ion channel activity Kir6.1 contributes critically to cellular homeostasis.

Pathways

Kir6.1 channels integrate into several important biological systems. Notably the K_ATP channels participate in the cardiac muscle's ischemic conditioning pathway helping protect cells against nutrient deprivation. These channels also engage in the insulin signaling pathway affecting glucose homeostasis and energy metabolism. The interaction with other proteins like SUR2 and Kir6.2 indicates the complex role Kir6.1 plays in these signaling cascades and its indispensable function in metabolic regulation.

The Kir6.1 channel has associations with cardiovascular diseases such as hypertension due to its involvement in vascular tone regulation. Abnormalities in Kir6.1 function can also contribute to disorders like neonatal diabetes linked to defects in insulin secretion due to channel dysfunction. Kir6.1 has connections with the SUR2 protein in these conditions given their partnership in the K_ATP channel complex. Studying these associations helps understand Kir6.1's influence on disease development and potential therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it (PubMed : 20558321, PubMed : 21836131, PubMed : 24700710, PubMed : 28842488). Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages (PubMed : 20558321, PubMed : 21836131, PubMed : 24700710, PubMed : 28842488). The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium (PubMed : 20558321, PubMed : 21836131, PubMed : 24700710, PubMed : 28842488). Can form a sulfonylurea-sensitive but ATP-insensitive potassium channel with ABCC9 (By similarity).

See full target information KCNJ8

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Immunity, inflammation and disease 12:e1194 PubMed38501544

2024

Sodium butyrate activates the K channels to regulate the mechanism of Parkinson's disease microglia model inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Xu,Laofu Wen,Yunyi Tang,Zhenqiang Zhao,Miaojing Xu,Tan Wang,Zhibin Chen

Frontiers in pharmacology 14:1115543 PubMed37180726

2023

Immunohistochemical, pharmacovigilance, and omics analyses reveal the involvement of ATP-sensitive K channel subunits in cancers: role in drug-disease interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima Maqoud,Nicola Zizzo,Marcella Attimonelli,Antonella Tinelli,Giuseppe Passantino,Marina Antonacci,Girolamo Ranieri,Domenico Tricarico

Theranostics 12:6611-6625 PubMed36185602

2022

Kir6.1/K-ATP channel in astrocytes is an essential negative modulator of astrocytic pyroptosis in mouse model of depression.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Li,Si-Yuan Jiang,Tian Tian,Wen-Jie Li,You Xue,Ren-Hong Du,Gang Hu,Ming Lu

EMBO reports 22:e52389 PubMed34569705

2021

Single-cell transcriptomic landscape of cardiac neural crest cell derivatives during development.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Chen,Xuanyu Liu,Wenke Li,Huayan Shen,Ziyi Zeng,Kunlun Yin,James R Priest,Zhou Zhou

BioMed research international 2020:3039184 PubMed33134375

2020

ATP-Sensitive Potassium Channels Mediate the Cardioprotective Effect of against Myocardial Ischaemia-Reperfusion Injury and Inflammatory Reaction.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Ning,Li Jiang,Ting Hu,Xingyu Wang,Aihua Liu,Yimin Bao

Journal of cellular and molecular medicine : PubMed32757436

2020

Quantitative proteomics identified 3 oxidative phosphorylation genes with clinical prognostic significance in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Su,Fen-Fang Zhou,Tao Zhang,Dan-Wen Wang,Da Zhao,Xiao-Ming Hou,Mao-Hui Feng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kir6.1/KCNJ8 antibody [S366-60]

Immunocytochemistry/ Immunofluorescence - Anti-Kir6.1/KCNJ8 antibody [S366-60] (AB241996)

Immunocytochemistry/ Immunofluorescence - Anti-Kir6.1/KCNJ8 antibody [S366-60] (AB241996)

Western blot - Anti-Kir6.1/KCNJ8 antibody [S366-60] (AB241996)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (6)

Product promise