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AB122164

Anti-KLHL17 antibody

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Rabbit Polyclonal KLHL17 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KLHL17 aa 550 to C-terminus.

대체 명칭 보기

AF, KLHL17, Kelch-like protein 17, Actinfilin

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL17 antibody (AB122164)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL17 antibody (AB122164)

ab122164 at 1/100 dilution staining KLHL17 in Paraffin-embedded Human testis tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-KLHL17 antibody (AB122164)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KLHL17 antibody (AB122164)

Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122164 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-KLHL17 antibody (AB122164)
  • WB

Unknown

Western blot - Anti-KLHL17 antibody (AB122164)

All lanes:

Western blot - Anti-KLHL17 antibody (ab122164) at 1/250 dilution

Lane 1:

RT 4 lysate

Lane 2:

U 251 MG lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 70 kDa

true

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human KLHL17 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6TDP4

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보완 제품

Primary Antibodies

AB125247

Anti-GAPDH antibody [GA1R] - Loading Control

Lab Essentials

Western blot - Anti-GAPDH antibody [GA1R] - Loading Control (AB125247)

  • 5
  • 7
Primary Antibodies

AB231778

Anti-SP1 antibody [EPR22648-50] - ChIP Grade

20ul selling size|BOND RX™ Validated|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 antibody [EPR22648-50] - ChIP Grade (AB231778)

  • 0
  • 0
Primary Antibodies

AB229150

Anti-KLHL13 antibody - N-terminal

Western blot - Anti-KLHL13 antibody - N-terminal (AB229150)

  • 0
  • 0
Primary Antibodies

AB2480

Anti-MYL12A (phospho S19) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL12A (phospho S19) antibody (AB2480)

  • 5
  • 18
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

KLHL17 also known as Kelch-like protein 17 has a molecular mass of approximately 68 kDa. This protein belongs to the Kelch-like family characterized by the presence of a BTB/POZ and kelch domains. KLHL17 functions as an adaptor protein linking specific substrate proteins to the ubiquitin-proteasome system for targeted degradation. Expression of KLHL17 occurs mainly in the central nervous system.
Biological function summary

KLHL17 influences neuronal function and development. It forms part of an E3 ubiquitin ligase complex associating with Cullin 3 to facilitate protein ubiquitination. This complex plays a role in synaptic activity by regulating the levels of proteins critical for neuronal signaling. In the brain KLHL17 contributes to the fine-tuning of signaling pathways affecting cognition and memory.

Pathways

KLHL17 integrates into protein degradation and neurodevelopmental signaling pathways. It interacts with components of the ubiquitination pathway primarily linked with Cullin 3. By mediating substrate protein ubiquitination KLHL17 indirectly affects pathways like the Wnt signaling pathway essential for neuronal development. It also cooperates with proteins such as beta-catenin which participates in a wide range of cellular processes.

KLHL17 associates with neurodevelopmental and neurodegenerative diseases. Alterations in KLHL17 function may contribute to conditions such as Alzheimer's disease. Through its role in protein turnover and signaling modulation KLHL17 interacts with other proteins like amyloid precursor protein (APP) which may influence the pathological cascades associated with such disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Substrate-recognition component of some cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes. The BCR(KLHL17) complex mediates the ubiquitination and subsequent degradation of GLUR6. May play a role in the actin-based neuronal function (By similarity).
See full target information KLHL17
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Open medicine (Warsaw, Poland) 16:58-67 PubMed33364434

2020

SLC12A8 plays a key role in bladder cancer progression and EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Shun-Lai Li,Zheng-Feng Li,Qing-Wei Cao,Wen-Zhen Wang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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