Anti-Klotho antibody [EPR6856]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (AB181373)

Immunohistochemical analysis of paraffin-embedded human kidney clear cell carcinoma tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (AB181373)

Immunohistochemical staining of paraffin embedded human kidney with purified ab181373 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] (AB181373)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Klotho antibody [EPR6856] (AB181373)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Klotho antibody [EPR6856] (ab181373) at 1/1000 dilution

Lane 1:

Human fetal kidney at 20 µg

Lane 2:

Human testis at 20 µg

Lane 3:

Human fetal thymus at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Predicted band size: 116 kDa

Observed band size: 62 kDa

false

• WB

Supplier Data

Western blot - Anti-Klotho antibody [EPR6856] (AB181373)

All lanes:

Western blot - Anti-Klotho antibody [EPR6856] (ab181373) at 1/1000 dilution

Lane 1:

Human fetal kidney lysates at 10 µg

Lane 2:

Human testis lysates at 10 µg

Lane 3:

Human fetal thymus lysates at 10 µg

Predicted band size: 116 kDa

false

• WB

Lab

Western blot - Anti-Klotho antibody [EPR6856] (AB181373)

The blots were produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane were blocked for an hour before being incubated with ab181373 (rabbit anti-Klotho antibody; diluted 5 ug/ml) for 24 hours at 4°C. Antibody binding was detected using infrared labelled goat anti-rabbit (green) antibody at 1 : 10,000 dilutions for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Klotho antibody [EPR6856] (ab181373) at 5 µg/mL

Lane 1:

Wild Type (WT) Mouse Kidney Tissue Lysate at 20 µg

Lane 2:

Knock Out (KO) Mouse Kidney Tissue Lysate at 20 µg

Predicted band size: 116 kDa,38 kDa,84 kDa

Observed band size: 50 kDa,72 kDa

false