AB12317

Anti-KMT1E / SETDB1 antibody

Name: Anti-KMT1E / SETDB1 antibody
Brand: Abcam Limited
SKU: AB12317
Rating: 2 (4 reviews)

(4 리뷰들)

(32 제품이 사용된 논문 )

Rabbit Polyclonal KMT1E / SETDB1 antibody. Suitable for WB and reacts with Human samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human SETDB1 aa 1100 to C-terminus.

대체 명칭 보기

ESET, KIAA0067, KMT1E, SETDB1, Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, H3-K9-HMTase 4

2 이미지

CiteAb

Western blot - Anti-KMT1E / SETDB1 antibody (AB12317)

Western Blotting using Anti-KMT1E / SETDB1 antibody, ab12317. Publication image from Li, J. et al., 2015, Nucleic Acids Res, 26405197. Legend direct from paper.

Ribosomes have a SetDB1-dependent H3K9 methyltransferase activity. (A) Increasing amounts of ribosomes (0.2 and 0.6 ug of RNA) were analyzed by Western blot, as indicated. (B) Western blot analysis of samples derived from immunoprecipitation assays using 50 ug of ribosomal RNA with antibodies against SetDB1 and RPS3a, as well as rabbit antibodies, as negative control. (C) Western blot of HeLa cell extracts treated with 30 nM of either control or SetDB1 siRNA for 72 h, blotted as indicated. (D) Western blot of ribosomes purified by sucrose gradient from HeLa cells treated with 30 nM of either control or SetDB1 siRNA for 72 h, blotted as indicated. 50 ug of S100 cytosolic extract was loaded as a positive control. (E) Histone methyltransferase assay using 3H-SAM, 5 µM of histone H3 peptides from amino acids 1–20 that were either unmodified or trimethylated at the lysine 9, and ribosomes purified by sucrose gradient from HeLa cells treated with 30 nM of either control or SetDB1 siRNA for 72 h. H3K9 methylation value was obtained subtracting the cpms obtained in the reaction using H3(1–20)K9me3 peptide from the cpms obtained in the reaction containing the H3(1–20) peptide. Standard deviation was obtained from triplicates.

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CiteAb

Western blot - Anti-KMT1E / SETDB1 antibody (AB12317)

Western Blotting using Anti-KMT1E / SETDB1 antibody, ab12317. Publication image from Li, J. et al., 2015, Nucleic Acids Res, 26405197. Legend direct from paper.

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human SETDB1 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15047

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified using the peptide immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

KMT1E also known as SETDB1 is a lysine methyltransferase with a role in epigenetic regulation. This enzyme has a molecular weight of approximately 143 kDa. It actively methylates histone H3 at lysine 9 (H3K9) a modification associated with transcriptional repression. SETDB1 contains a SET domain responsible for its methyltransferase activity. Expressed mainly in the nucleus this enzyme plays a critical role in controlling gene expression across various tissues including the brain spleen and testis.

Biological function summary

The methyltransferase SETDB1 impacts chromatin structure and gene silencing. It operates as a part of the histone methylation complex collaborating with co-factors like ATF7IP and MBD1 to achieve highly specific gene repression. By catalyzing trimethylation of H3K9 it creates a binding site for HP1 proteins facilitating the formation of heterochromatin. This action is essential for processes such as X-chromosome inactivation and the repression of transposable elements.

Pathways

Regulation through SETDB1 influences key biological processes. This enzyme is integral to the PRC2 (Polycomb Repressive Complex 2) pathway maintaining transcriptional silencing during development. Furthermore SETDB1 works alongside SUV39H1 in dynamic change of heterochromatin states regulating genes involved in cell cycle and differentiation. Such pathways highlight its significant interactions with proteins like EZH2 and EED which assist in maintaining stable gene silencing.

SETDB1 shows a connection with cancer particularly in melanoma and lung cancer. Overexpression or dysregulation of SETDB1 can lead to aberrant methylation patterns contributing to oncogenesis through alteration of tumor suppressor genes. Additionally schizophrenia has an association with SETDB1 where improper histone modification can impact gene expression programs critical for neurological function. In these contexts proteins such as DNMT1 also play a role as they collaborate in epigenetic modifications linked to the disease development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3) (PubMed : 11959841, PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones (PubMed : 11959841, PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes (PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). H3 'Lys-9' trimethylation is coordinated with DNA methylation (PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (PubMed : 14536086). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1 (PubMed : 14536086). SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins (PubMed : 24623306). Required for HUSH-mediated heterochromatin formation and gene silencing (PubMed : 27732843). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (PubMed : 37938770). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed : 27029610). Also catalyzes mono- and dimethylation of 'Lys-9' of free histone H3 (H3K9me1 and H3K9me2) during translation in the cytoplasm, which is then transported to the nucleus and incorporated into nucleosomes (PubMed : 26405197).. Isoform 2. Lacks all domains required for histone methyltransferase activity.

See full target information SETDB1

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제품이 사용된 논문 (32)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cellular and infection microbiology 15:1537929 PubMed40270769

2025

Sirtuin 2 inhibitor AGK2 exerts antiviral effects by inducing epigenetic suppression of hepatitis B virus covalently closed circular DNA through recruitment of repressive histone lysine methyltransferases and reduction of cccDNA.

Applications

Unspecified application

Species

Unspecified reactive species

Jumi Kim,Jiseon Ha,Chanho Song,Muhammad Azhar Sajjad,Fadia Kalsoom,Hyeonjoong Kwon,Jaewoo Park,Sun Park,Kyongmin Kim

Epigenetics & chromatin 18:2 PubMed39800758

2025

H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts.

Applications

Unspecified application

Species

Unspecified reactive species

Wilhelm Bouchereau,Hong-Thu Pham,Worawalan Samruan,Van-Hong Vu,Thierry Joly,Marielle Afanassieff,Pierre Savatier,Rangsun Parnpai,Nathalie Beaujean

Cell reports 42:113414 PubMed37967011

2023

PAK1-dependent mechanotransduction enables myofibroblast nuclear adaptation and chromatin organization during fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Elliot Jokl,Aoibheann F Mullan,Kara Simpson,Lindsay Birchall,Laurence Pearmain,Katherine Martin,James Pritchett,Sayyid Raza,Rajesh Shah,Nigel W Hodson,Craig J Williams,Elizabeth Camacho,Leo Zeef,Ian Donaldson,Varinder S Athwal,Neil A Hanley,Karen Piper Hanley

Journal of immunology research 2022:4012920 PubMed35497876

2022

Histone Methyltransferase SETDB1 Promotes Immune Evasion in Colorectal Cancer FOSB-Mediated Downregulation of MicroRNA-22 through BATF3/PD-L1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiale Tian,Weiwei Wang,Jichao Zhu,Yun Zhuang,Chunrun Qi,Zhengxin Cai,Wenhui Yan,Wenying Lu,Anquan Shang

Molecular cell 82:816-832.e12 PubMed35081363

2022

SETDB1/NSD-dependent H3K9me3/H3K36me3 dual heterochromatin maintains gene expression profiles by bookmarking poised enhancers.

Applications

Unspecified application

Species

Unspecified reactive species

Amandine Barral,Gabrielle Pozo,Lucas Ducrot,Giorgio L Papadopoulos,Sandrine Sauzet,Andrew J Oldfield,Giacomo Cavalli,Jérôme Déjardin

The Journal of experimental medicine 219: PubMed34825915

2021

WEE1 inhibition induces anti-tumor immunity by activating ERV and the dsRNA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ensong Guo,Rourou Xiao,Yifan Wu,Funian Lu,Chen Liu,Bin Yang,Xi Li,Yu Fu,Zizhuo Wang,Yuan Li,Yuhan Huang,Fuxia Li,Xue Wu,Lixin You,Tianyu Qin,Yiling Lu,Xiaoyuan Huang,Ding Ma,Gordon B Mills,Chaoyang Sun,Gang Chen

ACS chemical biology 16:1721-1736 PubMed34415726

2021

A Peptidomimetic Ligand Targeting the Chromodomain of MPP8 Reveals HRP2's Association with the HUSH Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Jarod M Waybright,Sarah E Clinkscales,Kimberly D Barnash,Gabrielle R Budziszewski,Justin M Rectenwald,Anna M Chiarella,Jacqueline L Norris-Drouin,Stephanie H Cholensky,Kenneth H Pearce,Laura E Herring,Robert K McGinty,Nathaniel A Hathaway,Lindsey I James

PLoS pathogens 16:e1008834 PubMed32956422

2020

Genome-wide CRISPR knockout screen identifies ZNF304 as a silencer of HIV transcription that promotes viral latency.

Applications

Unspecified application

Species

Unspecified reactive species

Simona Krasnopolsky,Alona Kuzmina,Ran Taube

Journal of virology 94: PubMed32493816

2020

An Alternatively Spliced Sirtuin 2 Isoform 5 Inhibits Hepatitis B Virus Replication from cccDNA by Repressing Epigenetic Modifications Made by Histone Lysine Methyltransferases.

Applications

Unspecified application

Species

Unspecified reactive species

Zahra Zahid Piracha,Umar Saeed,Jumi Kim,Hyeonjoong Kwon,Yong-Joon Chwae,Hyun Woong Lee,Jin Hong Lim,Sun Park,Ho-Joon Shin,Kyongmin Kim

Cell 181:800-817.e22 PubMed32302590

2020

Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Michele M Nava,Yekaterina A Miroshnikova,Leah C Biggs,Daniel B Whitefield,Franziska Metge,Jorge Boucas,Helena Vihinen,Eija Jokitalo,Xinping Li,Juan Manuel García Arcos,Bernd Hoffmann,Rudolf Merkel,Carien M Niessen,Kris Noel Dahl,Sara A Wickström

제품이 사용된 논문 모두 보기

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Product promise

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Anti-KMT1E / SETDB1 antibody

Western blot - Anti-KMT1E / SETDB1 antibody (AB12317)

Western blot - Anti-KMT1E / SETDB1 antibody (AB12317)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (32)

Product promise