AB300518

Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal KMT5A / SETD8 / Pr-SET7 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), WB and reacts with Human samples.

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Supplier Data

Immunoprecipitation - Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] - BSA and Azide free (AB300518)

This data was developed using ab300518, the same antibody clone in a different buffer formulation. KMT5A / SETD8 / Pr-SET7 was immunoprecipitated from 0.35 mg U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate 10 ug with ab300517 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300517 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/1000 dilution. Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate 10 ug Lane 2 : ab300517 IP in U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300517 in U-2 OS whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes

Lane 1:

Immunoprecipitation - Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] (<a href='/ko/products/primary-antibodies/kmt5a-setd8-pr-set7-antibody-epr24381-24-ab300517'>ab300517</a>) at 1/30 dilution

Lane 2:

Immunoprecipitation - Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] (<a href='/ko/products/primary-antibodies/kmt5a-setd8-pr-set7-antibody-epr24381-24-ab300517'>ab300517</a>) at 1/1000 dilution

All lanes:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

false

Exposure time: 3min

Lab

Western blot - Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] - BSA and Azide free (AB300518)

This data was developed using ab300517, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab300517 was shown to bind specifically to N-lysine methyltransferase KMT5A. A band was observed at 43 kDa in wild-type HEK293 cell lysates whereas no signal observed at this size in N-lysine methyltransferase KMT5A knockout cell line ab261858 (knockout cell lysate ab261667).

All lanes:

Western blot - Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] (<a href='/ko/products/primary-antibodies/kmt5a-setd8-pr-set7-antibody-epr24381-24-ab300517'>ab300517</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

KMT5A knockout HEK-293 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 43 kDa

false

Exposure time: 147s

Lab

Western blot - Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] - BSA and Azide free (AB300518)

This data was developed using ab300517, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lanes 1-2 : 70 seconds; Lane 3 : 48 seconds

Lanes 1/2 were developed using a high sensitivity ECL substrate.

Lysates were freshly made and used immediately to minimize protein degradation.

All lanes:

Western blot - Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] (<a href='/ko/products/primary-antibodies/kmt5a-setd8-pr-set7-antibody-epr24381-24-ab300517'>ab300517</a>) at 1/1000 dilution

Lane 1:

HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 43 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24381-24

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

KMT5A also known as SETD8 or Pr-SET7 is a histone methyltransferase enzyme with a notable role in regulating chromatin dynamics. It specifically catalyzes the monomethylation of histone H4 at lysine 20 (H4K20me1) a modification linked to chromatin compaction and DNA damage response. The molecular mass of KMT5A is approximately 47 kDa. Expression of KMT5A is observed in various tissues including the liver kidney and to some extent in embryonic stem cells indicating its functionality across different stages of development and cell types.

Biological function summary

KMT5A's activity influences several essential cellular processes such as cell cycle progression and DNA repair. As part of a larger chromatin-modulating framework it often functions alongside other chromatin-associated proteins including those from the Polycomb group. It plays a significant role in maintaining genomic stability by marking specific regions of chromatin for certain functions thereby affecting transcription regulation and cellular growth.

Pathways

KMT5A integrates into critical biological mechanisms like the DNA damage response and cell cycle regulation. It links closely with pathways involving p53 and cyclin-dependent kinase (CDK) inhibitors. KMT5A’s histone modification activity influences proteins such as p21 and p16 within the DNA damage signaling network. Its regulatory role here is essential as it facilitates an environment conducive to the DNA repair machinery following genotoxic stress.

KMT5A connects to various pathological states notably cancer and neurodegenerative disorders. Misregulation of KMT5A activity is associated with tumorigenesis where altered levels of H4K20me1 can lead to abnormal cell proliferation. Additionally KMT5A interacts through pathways tied to protein like TP53 influencing cellular responses to stressors typically seen in oncogenesis. Similarly aberrant regulation has implications in cognitive impairments potentially through dysregulated methylation patterns affecting neuronal cell cycle re-entry.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins (PubMed : 12086618, PubMed : 12121615, PubMed : 15964846, PubMed : 17707234, PubMed : 27338793). Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1) (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599, PubMed : 27338793). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599). Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599). Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599). Involved in chromosome condensation and proper cytokinesis (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599). Nucleosomes are preferred as substrate compared to free histones (PubMed : 12086618, PubMed : 12121615, PubMed : 15200950, PubMed : 15933069, PubMed : 15933070, PubMed : 15964846, PubMed : 16517599). Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes (PubMed : 17707234). Plays a negative role in TGF-beta response regulation and a positive role in cell migration (PubMed : 23478445).

See full target information KMT5A

대체 명칭 보기

PRSET7, SET07, SET8, SETD8, KMT5A, N-lysine methyltransferase KMT5A, H4-K20-HMTase KMT5A, Histone-lysine N-methyltransferase KMT5A, Lysine N-methyltransferase 5A, Lysine-specific methylase 5A, PR/SET domain-containing protein 07, SET domain-containing protein 8, PR-Set7, PR/SET07

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] - BSA and Azide free

Immunoprecipitation - Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] - BSA and Azide free (AB300518)

Western blot - Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] - BSA and Azide free (AB300518)

Western blot - Anti-KMT5A / SETD8 / Pr-SET7 antibody [EPR24381-24] - BSA and Azide free (AB300518)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise