AB6038

Anti-KPNA3 antibody

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(21 제품이 사용된 논문 )

Goat Polyclonal KPNA3 antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human KPNA3 aa 500 to C-terminus.

3 이미지

Flow Cytometry - Anti-KPNA3 antibody (AB6038)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-KPNA3 antibody (AB6038)

Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-KPNA3 antibody (AB6038)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KPNA3 antibody (AB6038)

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Supplier Data

Western blot - Anti-KPNA3 antibody (AB6038)

Detected by chemiluminescence.

Lanes 1 - 2:

Western blot - Anti-KPNA3 antibody (ab6038) at 0.03 µg/mL

Lane 3:

Western blot - Anti-KPNA3 antibody (ab6038) at 0.01 µg/mL

Lane 1:

CaCo-2 cell lysate at 35 µg

Lane 2:

HEK293 cell lysate at 35 µg

Lane 3:

MCF-7 cell lysate at 35 µg

Predicted band size: 57 kDa

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주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human KPNA3 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00505

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

GenBank Accession Number – NP_002258.

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

KPNA3 also known as importin subunit alpha-4 is a protein that mediates the nuclear import of proteins by recognizing nuclear localization signals. Its molecular mass is approximately 58 kDa. KPNA3 is present in various tissues including brain liver and heart which suggests its importance in cellular functions across these organs.

Biological function summary

KPNA3 facilitates the transport of proteins into the nucleus serving as an adapter between cargo proteins and the importin beta subunit. KPNA3 is part of the importin complex which is important for nucleocytoplasmic transport. The efficiency of nuclear protein import depends on KPNA3's ability to bind and release cargo proteins effectively.

Pathways

KPNA3 integrates into the nucleocytoplasmic transport pathways and interacts significantly with importin beta. It participates in the regulation of gene expression by ensuring the proper localization of transcription factors. The protein is also connected to other members of the importin family such as KPNA1 and KPNA2 which similarly serve roles in nuclear import pathways.

KPNA3 has links to various conditions such as viral infections and cancer. The protein can interact with viral proteins to facilitate the transport of viral genomes into the nucleus. In cancer KPNA3 expression levels often correlate with the deregulation of nuclear import pathways influencing tumor progression. KPNA3's interactions with proteins like p53 highlight its relevance in understanding disease mechanisms.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9.

See full target information KPNA3

대체 명칭 보기

QIP2, KPNA3, Importin subunit alpha-4, Importin alpha Q2, Karyopherin subunit alpha-3, SRP1-gamma, Qip2

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제품이 사용된 논문 (21)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of cell biology 224: PubMed39621428

2024

KPNA3 regulates histone locus body formation by modulating condensation and nuclear import of NPAT.

Applications

Unspecified application

Species

Unspecified reactive species

Shui Bo Xu,Xiu Kui Gao,Hao Di Liang,Xiao Xia Cong,Xu Qi Chen,Wen Kai Zou,Jia Li Tao,Zhao Yuan Pan,Jiao Zhao,Man Huang,Zhang Bao,Yi Ting Zhou,Li Ling Zheng

Virulence 12:754-765 PubMed33616472

2021

Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4.

Applications

Unspecified application

Species

Unspecified reactive species

Quan Xie,Weikang Wang,Luyuan Li,Qiuqi Kan,Hui Fu,Tuoyu Geng,Tuofan Li,Zhimin Wan,Wei Gao,Hongxia Shao,Aijian Qin,Jianqiang Ye

Cell reports 31:107549 PubMed32320654

2020

Cellular Importin-α3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Swantje Thiele,Stephanie Stanelle-Bertram,Sebastian Beck,Nancy Mounogou Kouassi,Martin Zickler,Martin Müller,Berfin Tuku,Patricia Resa-Infante,Debby van Riel,Malik Alawi,Thomas Günther,Franziska Rother,Stefanie Hügel,Susanne Reimering,Alice McHardy,Adam Grundhoff,Wolfram Brune,Albert Osterhaus,Michael Bader,Enno Hartmann,Gülsah Gabriel

International immunopharmacology 74:105575 PubMed31299609

2019

The selective NLRP3-inflammasome inhibitor MCC950 reduces myocardial fibrosis and improves cardiac remodeling in a mouse model of myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Rifeng Gao,Huairui Shi,Suchi Chang,Yang Gao,Xiao Li,Chunyu Lv,Heng Yang,Haiyan Xiang,Juesheng Yang,Lei Xu,Yanhua Tang

Cell reports 24:2213-2220 PubMed30157418

2018

Affinity for DNA Contributes to NLS Independent Nuclear Localization of MeCP2.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Lyst,Robert Ekiert,Jacky Guy,Jim Selfridge,Martha V Koerner,Cara Merusi,Dina De Sousa,Adrian Bird

Stem cell research & therapy 8:264 PubMed29141683

2017

Pellet coculture of osteoarthritic chondrocytes and infrapatellar fat pad-derived mesenchymal stem cells with chitosan/hyaluronic acid nanoparticles promotes chondrogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Huang,Xiongbo Song,Tao Li,Jingfang Xiao,Yemiao Chen,Xiaoyuan Gong,Weinan Zeng,Liu Yang,Cheng Chen

The Journal of biological chemistry 290:22485-93 PubMed26245896

2015

Karyopherin α 3 and karyopherin α 4 proteins mediate the nuclear import of methyl-CpG binding protein 2.

Applications

Unspecified application

Species

Unspecified reactive species

Steven Andrew Baker,Laura Marie Lombardi,Huda Yahya Zoghbi

Proteomics 15:1995-2005 PubMed25758553

2015

Proteomic identification of nuclear processes manipulated by cytomegalovirus early during infection.

Applications

Unspecified application

Species

Unspecified reactive species

Dominique M Carter,Kristen Westdorp,Kathleen R Noon,Scott S Terhune

The Journal of cell biology 205:301-12 PubMed24821838

2014

The p53-induced factor Ei24 inhibits nuclear import through an importin β-binding-like domain.

Applications

Unspecified application

Species

Unspecified reactive species

Kim G Lieu,Eun-Hee Shim,Jinling Wang,Ravi K Lokareddy,Tao Tao,Gino Cingolani,Gerard P Zambetti,David A Jans

FASEB journal : official publication of the Federa 28:3480-93 PubMed24790034

2014

The α-importome of mammalian germ cell maturation provides novel insights for importin biology.

Applications

Unspecified application

Species

Unspecified reactive species

Arash Arjomand,Mark A Baker,Chen Li,Ashley M Buckle,David A Jans,Kate L Loveland,Yoichi Miyamoto

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KPNA3 antibody

Flow Cytometry - Anti-KPNA3 antibody (AB6038)

Immunocytochemistry/ Immunofluorescence - Anti-KPNA3 antibody (AB6038)

Western blot - Anti-KPNA3 antibody (AB6038)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (21)

Product promise