AB316267

Anti-KRAS 4B isoform antibody [EPR26089-79]

20ul selling size
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal RASK antibody. Suitable for WB and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

대체 명칭 보기

KRAS2, RASK2, KRAS, GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras

3 이미지

Supplier Data

Western blot - Anti-KRAS 4B isoform antibody [EPR26089-79] (AB316267)

The identity of the bands over 25 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1-4 : 180 seconds; Lane 5 : 92 seconds

All lanes:

Western blot - Anti-KRAS 4B isoform antibody [EPR26089-79] (ab316267) at 1/1000 dilution

Lane 1:

Capan-1 (human pancreatic adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 50 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 50 µg

Lane 4:

C6 (rat glial tumor glial cell) whole cell lysate at 50 µg

Lane 5:

Human hippocampus tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 21 kDa,36 kDa

false

Supplier Data

Western blot - Anti-KRAS 4B isoform antibody [EPR26089-79] (AB316267)

The identity of the bands over 37 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-KRAS 4B isoform antibody [EPR26089-79] (ab316267) at 1/1000 dilution

Lane 1:

SW620 (human colorectal adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

SW620 transfected with siRNA specifically targeting KRAS 4B whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 21 kDa,36 kDa

false

Exposure time: 132s

Supplier Data

Western blot - Anti-KRAS 4B isoform antibody [EPR26089-79] (AB316267)

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-KRAS 4B isoform antibody [EPR26089-79] (ab316267) at 1/1000 dilution

Lane 1:

His-tagged human KRAS 4B recombinant protein at 20 ng

Lane 2:

His-tagged human KRAS 4A recombinant protein at 20 ng

Lane 3:

His-tagged human NRAS recombinant protein at 20 ng

Lane 4:

His-tagged human HRAS recombinant protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa

false

Exposure time: 92s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26089-79

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recommend to load 40-50 micrograms per lane to see a clear signal.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

KRAS 4B isoform also known as K-Ras4B or simply KRAS acts as a small GTPase a type of enzyme that helps in the conversion of guanosine triphosphate (GTP) to guanosine diphosphate (GDP). KRAS 4B is approximately 21 kDa in mass. It functions by cycling between an active GTP-bound state and an inactive GDP-bound state. Expression of KRAS 4B is found mainly in the epithelial cells and it is especially abundant in the lungs colon and pancreas. Its localization is significant for its role in signaling and regulation of cellular functions.

Biological function summary

KRAS 4B isoform plays an essential role in signal transduction processes that govern cell growth differentiation and survival. It operates within complex signaling networks but does not bind with a specific complex itself. KRAS 4B is part of the Ras superfamily of proteins which are pivotal in the transduction of signals from cell surface receptors to the nucleus. Its activity influences many cellular outcomes and dysregulation can lead to aberrant cellular processes.

Pathways

KRAS 4B is critically involved in the MAPK (Mitogen-Activated Protein Kinase) and PI3K/AKT (Phosphoinositide 3-kinase/Protein Kinase B) pathways which are key in cell proliferation and survival. KRAS 4B interacts closely with other proteins such as RAF and PI3K serving as a nexus for transmitting extracellular signals to intracellular effectors. These pathways facilitate diverse biological outcomes depending on the context and cellular environment.

KRAS 4B holds a strong connection with several types of cancer particularly colorectal and pancreatic cancers. Mutations in the KRAS gene can lead to constitutive activation of the protein which promotes uncontrolled cell division and tumor growth. In these contexts KRAS 4B often interacts with other proteins like SOS (Son of Sevenless) and RAF to propagate the oncogenic signaling cascades. Therapeutic targeting of the KRAS pathway remains a challenging but vital area in cancer treatment research.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Signal transducer in the Ras-MAPK signaling pathway that regulates cell proliferation and survival (PubMed : 22711838, PubMed : 23698361). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed : 20949621, PubMed : 39809765). Activates MAPK1/MAPK3 resulting in phosphorylation and ultimately degradation of GJA1 (By similarity). Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner (PubMed : 24623306). Recognized by LZTR1 that mediates its ubiquitination by a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex (PubMed : 40934300).

See full target information KRAS

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KRAS 4B isoform antibody [EPR26089-79]

Western blot - Anti-KRAS 4B isoform antibody [EPR26089-79] (AB316267)

Western blot - Anti-KRAS 4B isoform antibody [EPR26089-79] (AB316267)

Western blot - Anti-KRAS 4B isoform antibody [EPR26089-79] (AB316267)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise