AB196025

Anti-KRIT1 antibody [EPR16560]

Name: Anti-KRIT1 antibody [EPR16560]
Brand: Abcam Limited
SKU: AB196025
Rating: 5 (3 reviews)

RabMAb
Recombinant
각 태그의 뜻

(3 리뷰들)

(11 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal KRIT1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 11 publications.

대체 명칭 보기

CCM1, KRIT1, Krev interaction trapped protein 1, Krev interaction trapped 1, Cerebral cavernous malformations 1 protein

4 이미지

Immunocytochemistry/ Immunofluorescence - Anti-KRIT1 antibody [EPR16560] (AB196025)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KRIT1 antibody [EPR16560] (AB196025)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (Human umbilical vein endothelial cell line) cells labeling KRIT1 with ab196025 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on HUVEC cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab196025 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

Supplier Data

Western blot - Anti-KRIT1 antibody [EPR16560] (AB196025)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/5000 dilution

All lanes:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

Supplier Data

Western blot - Anti-KRIT1 antibody [EPR16560] (AB196025)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/5000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

SH-SY5Y (Human neuroblastoma from bone marrow cells) cell lysate at 20 µg

Lane 3:

Raji (Human Burkitt's lymphoma cell line) cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

Supplier Data

Western blot - Anti-KRIT1 antibody [EPR16560] (AB196025)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KRIT1 antibody [EPR16560] (ab196025) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

NIH/3T3 (Mouse embyro fibroblast cells) cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa

Observed band size: 84 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16560

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

KRIT1 also known as Krev interaction trapped 1 and CCM1 is a protein encoded by the KRIT1 gene. This protein has a mass of approximately 99 kilodaltons. Mechanically KRIT1 functions as an adapter protein interacting with several molecular partners to regulate cellular processes. KRIT1 is expressed broadly in various tissues including in the endothelial cells of blood vessels.

Biological function summary

This protein is involved in the maintenance of vascular integrity and formation. It forms a complex with other proteins like CCM2 and PDCD10 playing a critical role in stabilizing cell-cell junctions. By organizing junctional proteins KRIT1 supports cellular architecture and mechanotransduction. This action ensures proper response to hemodynamic forces within blood vessels.

Pathways

KRIT1 is involved in the RhoA signaling pathway and integrin-dependent pathways. In the RhoA signaling pathway KRIT1 interacts with HEG1 and MAPK to facilitate signal transduction that influences cell shape and movement. Through the integrin-dependent pathway KRIT1 regulates integrins essential for cell adhesion and migration. This regulation highlights its role in mechanotransduction and vascular stability.

KRIT1 mutations associate with cerebral cavernous malformations (CCM) a condition characterized by vascular lesions in the brain. These mutations disrupt interactions with CCM2 affecting vascular development and stability. KRIT1 has connections to other cerebrovascular-related proteins indicating its involvement in leaky blood vessels that form lesions. Understanding KRIT1's interaction pathways is key to developing therapies for CCM and potentially related vascular disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity (By similarity). Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits ERK1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction, and cell junction stabilization. Plays a role in integrin signaling via its interaction with ITGB1BP1; this prevents the interaction between ITGB1 and ITGB1BP1. Microtubule-associated protein that binds to phosphatidylinositol 4,5-bisphosphate (PIP2)-containing membranes in a GTP-bound RAP1-dependent manner. Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin-D1 (CCND1) levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels. May play a role in the regulation of macroautophagy through the down-regulation of the mTOR pathway (PubMed : 26417067).

See full target information KRIT1

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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of cell biology 223: PubMed39013281

2024

TLNRD1 is a CCM complex component and regulates endothelial barrier integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Neil J Ball,Sujan Ghimire,Gautier Follain,Ada O Pajari,Diana Wurzinger,Monika Vaitkevičiūtė,Alana R Cowell,Bence Berki,Johanna Ivaska,Ilkka Paatero,Benjamin T Goult,Guillaume Jacquemet

APL bioengineering 8:016108 PubMed38352162

2024

Hyaluronic acid turnover controls the severity of cerebral cavernous malformations in bioengineered human micro-vessels.

Applications

Unspecified application

Species

Unspecified reactive species

Teodor E Yordanov,Mikaela S Keyser,Marco A Enriquez Martinez,Tyron Esposito,Juliann B Tefft,Elysse K Morris,Larisa I Labzin,Samantha J Stehbens,Alan E Rowan,Benjamin M Hogan,Christopher S Chen,Jan Lauko,Anne K Lagendijk

Circulation 149:1183-1201 PubMed38099436

2023

HEG1 Protects Against Atherosclerosis by Regulating Stable Flow-Induced KLF2/4 Expression in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ian A Tamargo,Kyung In Baek,Chenbo Xu,Dong Won Kang,Yerin Kim,Aitor Andueza,Darian Williams,Catherine Demos,Nicolas Villa-Roel,Sandeep Kumar,Christian Park,Rachel Choi,Janie Johnson,Seowon Chang,Paul Kim,Sheryl Tan,Kiyoung Jeong,Shoutaro Tsuji,Hanjoong Jo

Biomedicines 11: PubMed36831015

2023

Multidrug-Loaded Lipid Nanoemulsions for the Combinatorial Treatment of Cerebral Cavernous Malformation Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Perrelli,Annalisa Bozza,Chiara Ferraris,Sara Osella,Andrea Moglia,Silvia Mioletti,Luigi Battaglia,Saverio Francesco Retta

Biomaterials science 8:5133-5144 PubMed32821891

2020

NIR-II window tracking of hyperglycemia induced intracerebral hemorrhage in cerebral cavernous malformation deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Abdul K Parchur,Zhi Fang,Jaidip M Jagtap,Gayatri Sharma,Christopher Hansen,Shayan Shafiee,Wenquan Hu,Qing R Miao,Amit Joshi

Methods in molecular biology (Clifton, N.J.) 2152:131-137 PubMed32524549

2020

Generation of CCM Phenotype by a Human Microvascular Endothelial Model.

Applications

Unspecified application

Species

Unspecified reactive species

Simona Delle Monache,Saverio Francesco Retta

Methods in molecular biology (Clifton, N.J.) 2152:151-167 PubMed32524551

2020

Production of KRIT1-knockout and KRIT1-knockin Mouse Embryonic Fibroblasts as Cellular Models of CCM Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Goitre,Claudia Fornelli,Alessia Zotta,Andrea Perrelli,Saverio Francesco Retta

Antioxidants (Basel, Switzerland) 8: PubMed30658464

2019

KRIT1 Loss-Of-Function Associated with Cerebral Cavernous Malformation Disease Leads to Enhanced -Glutathionylation of Distinct Structural and Regulatory Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Cianfruglia,Andrea Perrelli,Claudia Fornelli,Alessandro Magini,Stefania Gorbi,Anna Maria Salzano,Cinzia Antognelli,Francesca Retta,Valerio Benedetti,Paola Cassoni,Carla Emiliani,Giovanni Principato,Andrea Scaloni,Tatiana Armeni,Saverio Francesco Retta

ACS omega 3:15389-15398 PubMed30556006

2018

Multifunctional Platinum@BSA-Rapamycin Nanocarriers for the Combinatorial Therapy of Cerebral Cavernous Malformation.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa De Luca,Deborah Pedone,Mauro Moglianetti,Daniele Pulcini,Andrea Perrelli,Saverio Francesco Retta,Pier Paolo Pompa

Human mutation 39:1885-1900 PubMed30161288

2018

A single-center study on 140 patients with cerebral cavernous malformations: 28 new pathogenic variants and functional characterization of a PDCD10 large deletion.

Applications

Unspecified application

Species

Unspecified reactive species

Grazia Nardella,Grazia Visci,Vito Guarnieri,Stefano Castellana,Tommaso Biagini,Luigi Bisceglia,Orazio Palumbo,Marina Trivisano,Carmela Vaira,Massimo Scerrati,Davide Debrasi,Vincenzo D'Angelo,Massimo Carella,Giuseppe Merla,Tommaso Mazza,Marco Castori,Leonardo D'Agruma,Carmela Fusco

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KRIT1 antibody [EPR16560]

Immunocytochemistry/ Immunofluorescence - Anti-KRIT1 antibody [EPR16560] (AB196025)

Western blot - Anti-KRIT1 antibody [EPR16560] (AB196025)

Western blot - Anti-KRIT1 antibody [EPR16560] (AB196025)

Western blot - Anti-KRIT1 antibody [EPR16560] (AB196025)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (11)

Product promise