AB254279

Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free

BOND RX™ Validated
Recombinant
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Mouse Recombinant Monoclonal Kv4.3/KCND3 antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat samples.

대체 명칭 보기

A-type voltage-gated potassium channel KCND3, Potassium voltage-gated channel subfamily D member 3, Voltage-gated potassium channel subunit Kv4.3, Kcnd3

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (AB254279)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (AB254279)

This data was developed using ab252538, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the mouse cerebrum. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (AB254279)

This data was developed using ab252538, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on the mouse skeletal muscle. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (AB254279)

This data was developed using ab252538, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on the rat skeletal muscle. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (AB254279)

This data was developed using ab252538, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the rat cerebrum. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

K75/41

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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제품 세부 정보

ab254279 is the carrier-free version of ab252538.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Kv4.3 also known as KCND3 is a voltage-gated potassium channel subunit with an important role in membrane repolarization. This protein with a mass of approximately 71 kDa is extensively expressed in the heart and brain. Kv4.3 contributes to the formation of transient outward potassium currents (Ito) that are essential for shaping the action potentials in neurons and cardiac myocytes. These currents help in the rapid repolarization phase that restores the resting membrane potential after depolarization.

Biological function summary

Kv4.3 influences electrical signaling and cellular excitability in the cardiovascular and nervous systems. It often forms complexes with auxiliary subunits such as KChIP1-4 (Kv channel-interacting proteins) which modulate its current properties and localization. In neurons Kv4.3 helps regulate firing patterns while in cardiac tissue it affects heart rate and contractility. The presence of these complexes ensures precise control of channel function fitting the specific needs of tissue type and physiological condition.

Pathways

Kv4.3 integrates into critical signaling networks that involve ion homeostasis and action potential propagation. It belongs to the pathway that manages membrane potential dynamics interacting with proteins like Kv4.2 and Kv2.1 as part of a broader set of voltage-gated potassium channels. This network plays a role in the Wnt signaling pathway impacting cellular processes such as cell cycle regulation and differentiation.

Kv4.3 has been linked with cardiac arrhythmias and neurological disorders. Altered Kv4.3 functionality can lead to improper cardiac electrical activity resulting in arrhythmic events such as Long QT Syndrome. In the brain aberrations in Kv4.3 channel activity can correlate with epilepsy where disrupted ion channel regulation triggers excessive neuronal firing. Its involvement in these conditions highlights its participation in concert with other related proteins like SCN5A in cardiac tissues and CACNA1H in neuronal contexts.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Pore-forming (alpha) subunit of voltage-gated A-type potassium channels that mediates transmembrane potassium transport in excitable membranes, in brain and heart (By similarity). In cardiomyocytes, may generate the transient outward potassium current I(To). In neurons, may conduct the transient subthreshold somatodendritic A-type potassium current (ISA) (By similarity). Kinetics properties are characterized by fast activation at subthreshold membrane potentials, rapid inactivation, and quick recovery from inactivation. Channel properties are modulated by interactions with regulatory subunits. Interaction with the regulatory subunits KCNIP1 or KCNIP2 modulates the channel gating kinetics namely channel activation and inactivation kinetics and rate of recovery from inactivation. Likewise, interaction with DPP6 modulates the channel gating kinetics namely channel activation and inactivation kinetics (By similarity).

See full target information Kcnd3

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (AB254279)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (AB254279)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (AB254279)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free (AB254279)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise