AB240482

Anti-Lactate Dehydrogenase B/LDH-B antibody

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(5 제품이 사용된 논문 )

Goat Polyclonal L-lactate dehydrogenase B antibody. Suitable for WB and reacts with Pig, Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human LDHB aa 250 to C-terminus.

대체 명칭 보기

L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, Renal carcinoma antigen NY-REN-46, LDH-H, LDHB

2 이미지

Supplier Data

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (AB240482)

Primary incubation for 1 hour.

All lanes:

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482) at 0.1 µg/mL

Lane 1:

Human heart lysate in RIPA buffer at 35 µg

Lane 2:

Mouse heart lysate in RIPA buffer at 35 µg

Lane 3:

Rat heart lysate in RIPA buffer at 35 µg

Predicted band size: 21 kDa,37 kDa

true

Supplier Data

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (AB240482)

Primary incubation for 1 hour.

All lanes:

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482) at 0.1 µg/mL

All lanes:

Pig heart lysate in RIPA buffer at 35 µg

Predicted band size: 37 kDa

true

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

applications

Immunogen

Synthetic Peptide within Human LDHB aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P07195

Specificity

Reported variants represent identical protein: NP_002291.1, NP_001167568.1.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Lactate Dehydrogenase B (LDH-B) also known as LDH is an enzyme that plays an important role in the interconversion of lactate to pyruvate. It belongs to the lactate dehydrogenase family which includes multiple isoenzymes. LDH-B has a molecular weight of approximately 36 kDa and commonly forms a tetramer with three other similar subunits. It is mainly expressed in heart and muscle tissues among others where it functions effectively.

Biological function summary

LDH-B is essential for cellular energy production processes. It catalyzes the conversion of pyruvate a byproduct of glycolysis into lactate while simultaneously converting NADH to NAD+. This reaction is significant under anaerobic conditions where mitochondria-based ATP production is limited. LDH-B does not function alone and can operate as part of the lactate dehydrogenase complex containing different subunit combinations.

Pathways

Lactate and pyruvate metabolism engage LDH-B facilitating the Cori cycle and the lactate shuttle. These pathways enable lactate transport between tissues such as muscle and liver and they are important in maintaining blood glucose levels and energy balance. LDH-B operates alongside its related isoenzymes such as LDH-A which predominantly act in specific tissues and conditions to optimize energy utilization.

Anomalous LDH-B activity associates with conditions like myocardial infarction and some cancer types. Enhanced expression or activity of LDH-B can indicate altered metabolic states in tumors connecting it as a potential marker for certain cancerous conditions. The relationship with other proteins such as the hypoxia-inducible factor (HIF) is notable especially in cancer where HIF can regulate LDH-B expression under low oxygen environments.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

See full target information LDHB

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Stem cell research & therapy 15:415 PubMed39533309

2024

Lactylation of Hdac1 regulated by Ldh prevents the pluripotent-to-2C state conversion.

Applications

Unspecified application

Species

Unspecified reactive species

Qiman Dong,Xiaoqiong Yang,Lingling Wang,Qingye Zhang,Nannan Zhao,Shanshan Nai,Xiaoling Du,Lingyi Chen

iScience 27:110462 PubMed39104405

2024

SHP2 mutations promote glycolysis and inhibit apoptosis via PKM2/hnRNPK signaling in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhou,Zhuoyang Fan,Guodong He,Wei Zhang,Guowei Yang,Lechi Ye,Jianmin Xu,Rong Liu

International journal of molecular sciences 25: PubMed38732272

2024

Retinoic Acid-Mediated Control of Energy Metabolism Is Essential for Lung Branching Morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hugo Fernandes-Silva,Marco G Alves,Marcia R Garcez,Jorge Correia-Pinto,Pedro F Oliveira,Catarina C F Homem,Rute S Moura

International journal of molecular sciences 24: PubMed37446100

2023

Polymer-Functionalized Mitochondrial Transplantation to Fibroblasts Counteracts a Pro-Fibrotic Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Gherardo Baudo,Suhong Wu,Matteo Massaro,Haoran Liu,Hyunho Lee,Aijun Zhang,Dale J Hamilton,Elvin Blanco

Oncology letters 18:1431-1441 PubMed31423208

2019

Correlation and expression analysis of hypoxia-inducible factor 1α, glucose transporter 1 and lactate dehydrogenase 5 in human gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lang-Song Hao,Qi Liu,Chuan Tian,Dong-Xing Zhang,Bo Wang,Dong-Xu Zhou,Zhao-Peng Li,Zhi-Xiang Yuan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lactate Dehydrogenase B/LDH-B antibody

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (AB240482)

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (AB240482)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise