AB244455

Anti-LACTB antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal LACTB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LACTB aa 250-350.

대체 명칭 보기

MRPL56, UNQ843/PRO1781, LACTB

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB antibody (AB244455)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB antibody (AB244455)

Formalin-fixed, paraffin-embedded human liver tissue stained for LACTB with ab244455 at a 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-LACTB antibody (AB244455)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LACTB antibody (AB244455)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for LACTB (green) using ab244455 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB antibody (AB244455)

Formalin-fixed, paraffin-embedded human kidney tissue stained for LACTB with ab244455 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB antibody (AB244455)

Formalin-fixed, paraffin-embedded human testis tissue stained for LACTB with ab244455 at a 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB antibody (AB244455)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for LACTB with ab244455 at a 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-LACTB antibody (AB244455)

Western blot analysis labeling LACTB with ab244455 at 0.4 μg/mL.

All lanes:

Western blot - Anti-LACTB antibody (ab244455) at 0.4 µg/mL

All lanes:

RT-4 cell lysate

Predicted band size: 61 kDa

false

Unknown

Western blot - Anti-LACTB antibody (AB244455)

Western blot analysis labeling LACTB with ab244455 at 0.4 μg/mL.

All lanes:

Western blot - Anti-LACTB antibody (ab244455) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 61 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LACTB aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P83111

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The LACTB protein also known as Serine beta-lactamase-like protein LACTB functions primarily as a serine beta-lactamase-like protein involved in lipid metabolism. It is a mitochondrial protein with a molecular mass of approximately 61 kDa. LACTB is highly expressed in tissues such as the liver and kidney where its role in metabolic and oxidative processes is significant.

Biological function summary

LACTB has a critical function in mitochondrial maintenance and regulation. This protein is a part of the inner mitochondrial membrane and contributes to the regulation of mitochondrial lipid composition and mitochondrial morphology. It does not form a complex but interacts with several mitochondrial proteins to exert its functions. By adjusting the lipid makeup of the mitochondria LACTB influences various metabolic processes and cellular homeostasis.

Pathways

LACTB plays a significant role in oxidative phosphorylation and lipid metabolism pathways. In these pathways it interacts with various proteins that oversee lipid synthesis and mitochondrial function such as cardiolipin and fatty acid synthase. These interactions support energy production and the structural integrity of mitochondria which are essential for proper cellular metabolism and energy homeostasis.

LACTB has connections to cancer and metabolic syndrome. In cancer the protein's regulatory functions on mitochondrial lipid composition impact tumor growth and proliferation. Anomalies in LACTB expression have been associated with altered cellular metabolism observed in some cancers. In metabolic syndrome LACTB influences metabolic pathways altered in this disorder. In these conditions proteins like mTOR (mechanistic target of rapamycin) and AMPK (AMP-activated protein kinase) may interact with LACTB affecting cellular growth and metabolism regulation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Mitochondrial serine protease that acts as a regulator of mitochondrial lipid metabolism (PubMed : 28329758). Acts by decreasing protein levels of PISD, a mitochondrial enzyme that converts phosphatidylserine (PtdSer) to phosphatidylethanolamine (PtdEtn), thereby affecting mitochondrial lipid metabolism (PubMed : 28329758). It is unclear whether it acts directly by mediating proteolysis of PISD or by mediating proteolysis of another lipid metabolism protein (PubMed : 28329758). Acts as a tumor suppressor that has the ability to inhibit proliferation of multiple types of breast cancer cells : probably by promoting decreased levels of PISD, thereby affecting mitochondrial lipid metabolism (PubMed : 28329758).

See full target information LACTB

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell metabolism 37:154-168.e7 PubMed39561766

2024

Human genetics identify convergent signals in mitochondrial LACTB-mediated lipid metabolism in cardiovascular-kidney-metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Shen Li,Hongbo Liu,Hailong Hu,Eunji Ha,Praveena Prasad,Brenita C Jenkins,Ujjalkumar Subhash Das,Sarmistha Mukherjee,Kyosuke Shishikura,Renming Hu,Daniel J Rader,Liming Pei,Joseph A Baur,Megan L Matthews,Garret A FitzGerald,Melanie R McReynolds,Katalin Susztak

Molecular carcinogenesis 62:907-919 PubMed37157950

2023

PCBP1 protects bladder cancer cells from mitochondria injury and ferroptosis by inducing LACTB mRNA degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Luo,Yunli Zhang,Shiyu Pang,Jingxian Min,Tao Wang,Dali Wu,Chun Lin,Zebin Xiao,Qi Xiang,Qing Li,Lili Ma

Cancer science 114:1396-1409 PubMed36562402

2023

Circular RNA circ0104103 inhibits colorectal cancer progression through interactions with HuR and miR-373-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Tan,Huiling Sun,Mu Xu,Xiangxiang Liu,Jian Qin,Junjie Nie,Xiaodan Qin,Shukui Wang,Yuqin Pan

Oncology letters 21:470 PubMed33907580

2021

LACTB and LC3 could serve as potential biomarkers of gastric cancer to neoadjuvant chemotherapy with oxaliplatin plus S-1.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Yang,Zhiqiang Yan,Wei Nie,Zeying Liu,Xingzhen Cheng,Wei Wang,Chunyan Shao,Gui Fu,Yanni Yu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LACTB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB antibody (AB244455)

Immunocytochemistry/ Immunofluorescence - Anti-LACTB antibody (AB244455)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB antibody (AB244455)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB antibody (AB244455)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB antibody (AB244455)

Western blot - Anti-LACTB antibody (AB244455)

Western blot - Anti-LACTB antibody (AB244455)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Assay Kits

Assay Kits

Reagents, Controls & Accessories

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise