AB242197

Anti-LAMA3 antibody [CL3112]

(1 리뷰)

(7 제품이 사용된 논문 )

Mouse Monoclonal LAMA3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LAMA3 aa 2000 to C-terminus.

대체 명칭 보기

LAMNA, LAMA3, Laminin subunit alpha-3, Epiligrin 170 kDa subunit, Epiligrin subunit alpha, Kalinin subunit alpha, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, Nicein subunit alpha, E170

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Negative control :

Paraffin-embedded human lymph node tissue stained for LAMA3 using ab242197 at 1 μg/mL in immunohistochemcial analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Paraffin-embedded human colon tissue stained for LAMA3 using ab242197 at 1 μg/mL in immunohistochemcial analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Paraffin-embedded human stomach tissue stained for LAMA3 using ab242197 at 1 μg/mL in immunohistochemcial analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Paraffin-embedded human oral mucosa tissue stained for LAMA3 using ab242197 at 1 μg/mL in immunohistochemcial analysis.

Supplier Data

Western blot - Anti-LAMA3 antibody [CL3112] (AB242197)

All lanes:

Western blot - Anti-LAMA3 antibody [CL3112] (ab242197) at 1 µg/mL

Lane 1:

Purified human Lamin-332 recombinant protein

Lane 2:

Purified human Lamin-421 recombinant protein

Lane 3:

Purified human Lamin-511 recombinant protein

Lane 4:

Purified human Lamin-121 recombinant protein

Lane 5:

Purified human Lamin-221 recombinant protein

Predicted band size: 367 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3112

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human LAMA3 aa 2000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q16787

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p>Because LAMA3 expression levels can vary significantly across different tissue types, negative staining in IHC-P may result from low antigen expression. In cases where no signal is detected, using an HRP polymer-conjugated secondary antibody and DAB-based detection is recommended to enhance signal amplification and assay sensitivity.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Laminin subunit alpha-3 also known by the gene name LAMA3 is a critical component of the extracellular matrix. The protein has a molecular weight of approximately 400 kDa. LAMA3 is primarily expressed in epithelial tissues contributing significantly to the structural integrity of basement membranes. The protein often partners with other laminin subunits to form different laminin isoforms which are essential for cellular adhesion and signaling.

Biological function summary

LAMA3 plays an important role in the formation of stable basement membranes and interacts with other proteins to maintain tissue architecture. As part of the laminin-332 complex alongside beta and gamma laminin chains LAMA3 ensures cell adhesion migration and differentiation processes. Its expression in tissues like skin and mucosal surfaces highlights its function in providing structural support and cellular microenvironment regulation.

Pathways

Laminin subunit alpha-3 is essential in pathways related to cell adhesion and migration such as integrin signaling and epithelial-mesenchymal transition. These pathways are pivotal during wound healing and tissue regeneration. LAMA3 associates with integrins particularly beta-1 and beta-4 integrins facilitating communication between cells and their extracellular environment. The interaction with integrins enables cells to respond to structural changes and external stimuli.

Mutations or alterations in LAMA3 can lead to conditions such as Epidermolysis Bullosa and Junctional Epidermolysis Bullosa where the integrity of the skin and epithelial tissues is compromised. Laminin subunit gamma-2 often interacts with LAMA3 and anomalies in this partnership contribute to these severe blistering disorders. Understanding the link between LAMA3 and these diseases helps inform the development of targeted therapies aimed at restoring normal cell-matrix interactions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.. Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.

See full target information LAMA3

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Breast cancer research : BCR 26:187 PubMed39707454

2024

Cilengitide sensitivity is predicted by overall integrin expression in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nomeda Girnius,Aylin Z Henstridge,Benjamin Marks,Jeffrey K Yu,G Kenneth Gray,Chris Sander,Ioannis K Zervantonakis,Augustin Luna

Heliyon 10:e36234 PubMed39253230

2024

Comprehensive analysis and experimental verification of the mechanism of anoikis related genes in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Bao,Dongqian Li,Xinyu Yang,Shiqi Ren,Haoxiang Ding,Chengfeng Guo,Jian Wan,Yicheng Xiong,MingYan Zhu,Yao Wang

Analytical cellular pathology (Amsterdam) 2024:8810804 PubMed38826849

2024

FAP Serves as a Prognostic Biomarker in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanpeng Liao,Haidong Fan,Junquan Weng,Jieyu Zhou,Yuyan Zheng

Radiology and oncology 58:234-242 PubMed38452390

2024

Correlation of laminin subunit alpha 3 expression in pancreatic ductal adenocarcinoma with tumor liver metastasis and survival.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyi Xing,Xue Jing,Gong Qing,Yueping Jiang

Journal of ovarian research 14:67 PubMed33992120

2021

LAMA3 DNA methylation and transcriptome changes associated with chemotherapy resistance in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Yuan Feng,Yong-Zhi Huang,Wei Zhang,Li Li

Stem cells (Dayton, Ohio) 39:897-903 PubMed33609408

2021

ABCB5+ dermal mesenchymal stromal cells with favorable skin homing and local immunomodulation for recessive dystrophic epidermolysis bullosa treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Riedl,Michael Pickett-Leonard,Cindy Eide,Mark Andreas Kluth,Christoph Ganss,Natasha Y Frank,Markus H Frank,Christen L Ebens,Jakub Tolar

Molecular therapy. Nucleic acids 18:166-182 PubMed31557618

2019

Long Non-coding RNA LINC00628 Interacts Epigenetically with the LAMA3 Promoter and Contributes to Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Feng Xu,Yue Zheng,Ling Zhang,Ping Wang,Chun-Mi Niu,Tong Wu,Qi Tian,Xiao-Bo Yin,Shan-Shan Shi,Lei Zheng,Li-Ming Gao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMA3 antibody [CL3112]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Western blot - Anti-LAMA3 antibody [CL3112] (AB242197)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (7)

Product promise