AB150385

Anti-LAMB3 antibody [EPR7525]

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal LAMB3 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 3 publications.

대체 명칭 보기

LAMNB1, LAMB3, Laminin subunit beta-3, Epiligrin subunit beta, Kalinin B1 chain, Kalinin subunit beta, Laminin B1k chain, Laminin-5 subunit beta, Nicein subunit beta

1 이미지

Unknown

Western blot - Anti-LAMB3 antibody [EPR7525] (AB150385)

All lanes:

Western blot - Anti-LAMB3 antibody [EPR7525] (ab150385) at 1/1000 dilution

Lane 1:

HACAT cell lysate at 10 µg

Lane 2:

Raw264.7 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Gaot anti-Rabbit at 1/2000 dilution

Predicted band size: 130 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7525

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Laminin subunit beta-3 (LAMB3) is a protein that is part of the laminin family commonly referred to as Laminin-332 or Laminin-5. It weighs approximately 117 kDa. LAMB3 is expressed in the epithelial basement membranes of various tissues including skin and mucous membranes. The protein forms a network essential for maintaining the structural integrity of these membranes playing a mechanical role in cellular adhesion and migration.

Biological function summary

LAMB3 functions within a heterotrimeric complex that includes Laminin-3 (alpha subunit) and Laminin-3 (gamma subunit). These interactions lead to the formation of Laminin-332 which serves as a backbone for cell attachment and spreading. It is important in processes such as epithelial cell proliferation and wound healing. By stabilizing attachments between the basement membrane and epithelial cells LAMB3 supports tissue integrity and repair mechanisms.

Pathways

LAMB3 plays a role in both the integrin signaling and epidermal growth factor receptor (EGFR) pathways. It connects with proteins like the integrin alpha-6 beta-4 complex facilitating cell adhesion and communication with the extracellular matrix. In the EGFR pathway LAMB3's interactions enhance signaling that promotes cellular proliferation and survival playing a part in skin development and maintenance.

Mutations or dysregulations in LAMB3 relate notably to junctional epidermolysis bullosa and certain cancers like squamous cell carcinoma. In junctional epidermolysis bullosa the protein loses its adhesive function affecting the epithelial junctions and causing severe skin blistering. LAMB3 also associates with integrin proteins in cancer contributing to tumor progression and metastasis when dysfunctional affecting cell adhesion and integrity.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

See full target information LAMB3

style

left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 25: PubMed39201392

2024

TNF Induces Laminin-332-Encoding Genes in Endothelial Cells and Laminin-332 Promotes an Atherogenic Endothelial Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Assim Hayderi,Mulugeta Melkie Zegeye,Sare Meydan,Allan Sirsjö,Ashok Kumar Kumawat,Liza U Ljungberg

Investigative ophthalmology & visual science 58:2666-2684 PubMed28525556

2017

Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy A Whitson,Xiang Zhang,Mario Medvedovic,Jenny Chen,Zongbo Wei,Vincent M Monnier,Xingjun Fan

Molecular & cellular proteomics : MCP 14:1959-76 PubMed25953087

2015

Activating PIK3CA Mutations Induce an Epidermal Growth Factor Receptor (EGFR)/Extracellular Signal-regulated Kinase (ERK) Paracrine Signaling Axis in Basal-like Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Christian D Young,Lisa J Zimmerman,Daisuke Hoshino,Luigi Formisano,Ariella B Hanker,Michael L Gatza,Meghan M Morrison,Preston D Moore,Corbin A Whitwell,Bhuvanesh Dave,Thomas Stricker,Neil E Bhola,Grace O Silva,Premal Patel,Dana M Brantley-Sieders,Maren Levin,Marina Horiates,Norma A Palma,Kai Wang,Philip J Stephens,Charles M Perou,Alissa M Weaver,Joyce A O'Shaughnessy,Jenny C Chang,Ben Ho Park,Daniel C Liebler,Rebecca S Cook,Carlos L Arteaga

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMB3 antibody [EPR7525]

Western blot - Anti-LAMB3 antibody [EPR7525] (AB150385)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise