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AB236762

Anti-Laminin 2 alpha antibody [CL3450]

Anti-Laminin 2 alpha 항체 [CL3450]

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(4 제품이 사용된 논문 )

Anti-Laminin 2 alpha antibody [CL3450] (ab236762) is a mouse monoclonal antibody detecting Laminin 2 alpha in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

대체 명칭 보기

LAMM, LAMA2, Laminin subunit alpha-2, Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain

7 이미지
Western blot - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)
  • WB

Supplier Data

Western blot - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)

All lanes:

Western blot - Anti-Laminin 2 alpha antibody [CL3450] (ab236762) at 1 µg/mL

Lane 1:

Purified human recombinant Laminin-211

Lane 2:

Purified human recombinant Laminin-221

Lane 3:

Purified human recombinant Laminin-332

Lane 4:

Purified human recombinant Laminin-421

Lane 5:

Purified human recombinant Laminin-511

Lane 6:

Purified human recombinant Laminin-121

Predicted band size: 344 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)

Paraffin-embedded human placenta tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)

Paraffin-embedded human cerebral cortex tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)

Paraffin-embedded human heart tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)

Paraffin-embedded rat stomach tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)

Paraffin-embedded mouse heart tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [CL3450] (AB236762)

Paraffin-embedded rat heart tissue stained for Laminin 2 alpha using ab236762 at 1/200 dilution in immunohistochemical analysis.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3450

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human LAMA2 aa 2100-2250. The exact immunogen used to generate this antibody is proprietary information.

P24043

Epitope

Binds to an epitope located within the peptide sequence DKLKPIKELEDNLKK as determined by overlapping synthetic peptides.

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대체품

Primary Antibodies

AB140482

Anti-Laminin 2 alpha antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody (AB140482)

  • 0
  • 0
Primary Antibodies

AB11576

Anti-Laminin 2 alpha antibody [4H8-2]

Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [4H8-2] (AB11576)

  • 5
  • 8
Primary Antibodies

AB238458

Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR21890] - BSA and Azide free (AB238458)

  • 0
  • 0
Primary Antibodies

AB219409

Anti-Laminin 2 alpha antibody [EPR21890]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR21890] (AB219409)

  • 0
  • 0

보완 제품

Primary Antibodies

AB11575

Anti-Laminin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

  • 5
  • 74
Primary Antibodies

AB6586

Anti-Collagen IV antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (AB6586)

  • 5
  • 123
Primary Antibodies

AB7463

Anti-Laminin 1+2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Laminin 1+2 antibody (AB7463)

  • 3
  • 6
Primary Antibodies

AB275391

Anti-Dystrophin antibody [EPR23336-129]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] (AB275391)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

What is this antibody validated in?
Anti-Laminin 2 alpha antibody [CL3450] (ab236762) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of Laminin 2 alpha?
Anti-Laminin 2 alpha [CL3450] (ab236762) specifically detects a band for Laminin 2 alpha (UniProt: P24043) at a molecular weight of 344kDa.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
Purified from TCS
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Laminin 2 alpha also known as laminin alpha 2 or merosin is a subunit of the laminin protein family that contributes to the structure of the extracellular matrix. It forms part of the heterotrimeric laminin 211 complex comprising alpha beta and gamma chains. Laminin 2 alpha has a molecular weight of approximately 300 kDa and is expressed in various tissues especially in skeletal muscle and the peripheral nervous system. This subunit plays an important role in anchoring cells to the basement membrane facilitating cellular adhesion and communication.
Biological function summary

Laminin 2 alpha contributes to the integrity and function of the basement membrane by forming a scaffold that connects cells and tissues. It is part of the greater laminin molecule which contains other laminin proteins and helps in cell differentiation migration and adhesion. The protein serves as a binding site for various integrins and dystroglycans contributing to the development and repair processes within muscular and neural systems. It ensures stability in tissue architecture maintaining proper cell signaling pathways in collaboration with other extracellular matrix components.

Pathways

Laminin 2 alpha is involved in processes essential for tissue integrity and repair. It plays a role within the PI3K/AKT signaling pathway which is important for cell survival and metabolism. In this pathway laminin 2 alpha of the laminin 211 complex interacts with integrins and dystroglycan complexes facilitating downstream signaling for cellular growth and differentiation. Additionally laminin 2 alpha assists in the MAPK pathway connecting with proteins like FAK which regulates cell adhesion and proliferation important for healthy tissue function and response to damage.

Laminin 2 alpha is closely related to congenital muscular dystrophies particularly Merosin-deficient Congenital Muscular Dystrophy type 1A (MDC1A). This disorder is characterized by muscle weakness and developmental delays resulting from mutations in the laminin alpha 2 chain. Disruptions in laminin 2 can affect its interaction with the dystrophin-glycoprotein complex leading to compromised muscle membrane stability and tissue degeneration. Another connection exists with neuropathies where laminin 2 alpha's role in the peripheral nervous system can contribute to neural dysfunction if the protein is improperly expressed or mutated.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information LAMA2
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 14:6931 PubMed37903771

2023

Remodeling the cellular stress response for enhanced genetic code expansion in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mikhail E Sushkin,Christine Koehler,Edward A Lemke

BMC cancer 23:1021 PubMed37872487

2023

Identification of a basement membrane-related genes signature with immune correlation in bladder urothelial carcinoma and verification in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Yanze Li,Kai Xu,Ye Zhang,Hu Mao,Qiangmin Qiu,Zhiwei Yan,Xiuheng Liu,Yang Du,Zhiyuan Chen

Neuron 111:30-48.e14 PubMed36323321

2022

Mechanosensitive brain tumor cells construct blood-tumor barrier to mask chemosensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Chen,Ali Momin,Siyi Wanggou,Xian Wang,Hyun-Kee Min,Wenkun Dou,Zheyuan Gong,Jade Chan,Weifan Dong,Jerry J Fan,Yi Xiong,Kamilia Talipova,Hongyu Zhao,Yuki X Chen,Kelly Veerasammy,Adam Fekete,Sachin A Kumar,Hongwei Liu,Qi Yang,Joe Eun Son,Zhengchao Dou,Malini Hu,Parnian Pardis,Kyle Juraschka,Laura K Donovan,Jiao Zhang,Vijay Ramaswamy,Hayden J Selvadurai,Peter B Dirks,Michael D Taylor,Lu-Yang Wang,Chi-Chung Hui,Rinat Abzalimov,Ye He,Yu Sun,Xuejun Li,Xi Huang

Cell death & disease 11:614 PubMed32792503

2020

Mex3a interacts with LAMA2 to promote lung adenocarcinoma metastasis via PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Liang,Haixia Li,Jingyi Han,Jin Jiang,Jiang Wang,Yongmeng Li,Zitong Feng,Renchang Zhao,Zhenguo Sun,Bin Lv,Hui Tian
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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