Anti-Laminin 2 alpha antibody [EPR23924-60]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Laminin 2 alpha antibody [EPR23924-60] (AB259345)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse cardiac muscle tissue staining Connexin 43 / GJA1 with ab235585 at 0.507 μg/ml, ab308619 anti-MYBPC3, and ab259345 anti-Laminin subunit alpha-2.

Panel A : anti-Connexin 43 / GJA1 (green; Opal™520), anti-MYBPC3 (magenta; Opal™570), anti-Laminin subunit alpha-2 (gray; Opal™690) on mouse cardiac muscle.

Panel B : anti-Connexin 43 / GJA1 staining intercalated discs in mouse cardiac muscle.

Panel C : anti-MYBPC3 staining cytoplasm of heart myocytes in mouse cardiac muscle.

Panel D : anti-Laminin subunit alpha-2 staining basal laminae in mouse cardiac muscle.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab235585, ab308619 and ab259345 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] (AB259345)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle tissue labeling Laminin 2 alpha with ab259345 at 1/100 (4.84 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Green). Positive staining on mouse cardiac muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] (AB259345)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Laminin 2 alpha with ab259345 at 1/500 (0.484 μg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in basal laminae of mouse cardiac muscle (PMID : 11969289). The section was incubated with ab259345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin 2 alpha antibody [EPR23924-60] (AB259345)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Laminin 2 alpha with ab259345 at 1/500 (0.484 μg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in basal laminae of mouse skeletal muscle (PMID : 11969289). The section was incubated with ab259345 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.