Anti-Laminin gamma 1 antibody [EPR21199]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Laminin gamma 1 antibody [EPR21199] (AB233389)

Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized A431 (human epidermoid carcinoma cell line) cell line labeling Laminin gamma 1 with ab233389 at 1/50 (red) compared with Rabbit monoclonal IgG Isotype control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (AB233389)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in basement membrane of human pancreas (PMID : 15159456) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IP

Unknown

Immunoprecipitation - Anti-Laminin gamma 1 antibody [EPR21199] (AB233389)

Laminin gamma 1 was immunoprecipitated from 0.35 mg of A431 (human epidermoid carcinoma cell line) whole cell lysate with ab233389 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab233389 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : A431 whole lysate 10 μg (Input).
Lane 2 : ab233389 IP in A431 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab233389 in A431 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389)

Predicted band size: 178 kDa

Observed band size: 210-250 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (AB233389)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in basement membrane of rat stomach (PMID : 12571278) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21199] (AB233389)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Laminin gamma 1 with ab233389 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in the basement membrane of mouse kidney (PMID : 21903675) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• WB

Unknown

Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (AB233389)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1, 2 & 7 : 15 seconds. Lane 3 : 8 seconds. Lanes 4 : 37 seconds. Lanes 5 & 6 : 10 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 18263645, 11067943, 23857767, 28928891).

Lanes 1, 2, 3, 6 and 7:

Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/5000 dilution

Lanes 4 - 5:

Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 10 µg

Lane 2:

NIH:OVCAR-3 (human ovary adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 4:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 5:

A549 (human lung carcinoma cell line) whole cell lysate at 10 µg

Lane 6:

Human fetal heart lysate at 10 µg

Lane 7:

Human fetal kidney lysate at 10 µg

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 6 - 7:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 178 kDa

Observed band size: 210-250 kDa

false

• WB

Unknown

Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (AB233389)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times.

Lane 1 : 70 seconds. Lane 2 : 15 seconds. Lanes 3 & 4 : 10 seconds. Lanes 5 & 6 : 3 minutes. Lane 7 : 48 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 18263645, 11067943, 23857767, 28928891).

All lanes:

Western blot - Anti-Laminin gamma 1 antibody [EPR21199] (ab233389) at 1/5000 dilution

Lane 1:

C6 (rat glial tumor glial cell line) whole cell lysate at 10 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Lane 3:

Rat heart lysate at 10 µg

Lane 4:

Rat kidney lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Lane 6:

Rat spleen lysate at 10 µg

Lane 7:

Mouse brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 178 kDa

Observed band size: 210-250 kDa

false