Anti-Laminin gamma 1 antibody [EPR21200]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] (AB233390)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the basement membrane of human skin (PMID : 15159456) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] (AB233390)

Immunohistochemical analysis of paraffin-embedded human meningioma tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in human meningioma (PMID : 23053286) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] (AB233390)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the basement membrane of human kidney (PMID : 21903675) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• WB

Unknown

Western blot - Anti-Laminin gamma 1 antibody [EPR21200] (AB233390)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 10 seconds; Lanes 2-4 : 15 seconds; Lane 5 : 26 seconds; Lanes 6-7 : 6 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 18263645, 11067943, 23857767, 28928891).

All lanes:

Western blot - Anti-Laminin gamma 1 antibody [EPR21200] (ab233390) at 1/5000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Lane 3:

A431 (Human epidermoid carcinoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH:OVCAR-3 (Human ovary adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 5:

JAR (human placenta choriocarcinoma cell line) whole cell lysate at 20 µg

Lane 6:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 7:

A549 (human lung carcinoma cell line) whole cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Lanes 5 - 7:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 178 kDa

false