AB234959

Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal LAMC1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (AB234959)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (AB234959)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the basement membrane of human skin (PMID : 15159456) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233390).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (AB234959)

Immunohistochemical analysis of paraffin-embedded human meningioma tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in human meningioma (PMID : 23053286) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233390).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (AB234959)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Laminin gamma 1 with ab233390 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in the basement membrane of human kidney (PMID : 21903675) is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233390).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21200

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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제품 세부 정보

ab234959 is the carrier-free version of ab233390.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Laminin gamma 1 also known as laminin-1 is an important component of the extracellular matrix. This protein has a molecular weight of about 200 kDa and is widely expressed in various tissues including epithelial and endothelial cells. Laminin gamma 1 is part of the larger laminin protein family which plays a significant role in the structural integrity of tissues. It primarily partners with other laminin chains to form a heterotrimeric complex that provides foundational support for cellular structures.

Biological function summary

Laminin gamma 1 contributes to cell adhesion differentiation and migration. It belongs to the laminin-1 complex serving as a scaffold for cells to interact with each other and with the extracellular matrix. This interaction facilitates cell signaling processes that influence cellular behavior. Additionally the laminin iHC (immunohistochemistry) technique often utilizes laminin gamma 1 to study cell-matrix interactions due to its involvement in these critical biological activities.

Pathways

Laminin gamma 1 is integral to basement membrane assembly and epithelial morphogenesis pathways. These pathways incorporate other key proteins such as integrins which mediate cell attachment to the laminin matrix. The pathway interactions involving laminin gamma 1 allow cells to respond effectively to mechanical and chemical stimuli thereby maintaining tissue homeostasis and promoting regenerative processes after injury.

Abnormalities in laminin gamma 1 expression or function associate with conditions like muscular dystrophy and certain cancers. Mutations affecting this protein can lead to compromised structural integrity and impaired cellular communication. In muscular dystrophy its relationship with proteins such as dystroglycan highlights the disruption in muscle cell adhesion and signaling. Similarly in cancer altered laminin gamma 1 expression affects tumor cell invasion and metastasis involving interactions with proteins such as matrix metalloproteinases (MMPs) which regulate extracellular matrix remodeling.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. As a subunit of laminin-1 (also known as laminin-111 or EHS laminin), it is involved in the stimulation of agrin-induced receptor clustering through a MuSK-independent pathway.

See full target information LAMC1

대체 명칭 보기

LAMB2, LAMC1, Laminin subunit gamma-1, Laminin B2 chain, Laminin-1 subunit gamma, Laminin-10 subunit gamma, Laminin-11 subunit gamma, Laminin-2 subunit gamma, Laminin-3 subunit gamma, Laminin-4 subunit gamma, Laminin-6 subunit gamma, Laminin-7 subunit gamma, Laminin-8 subunit gamma, Laminin-9 subunit gamma, S-laminin subunit gamma, S-LAM gamma

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

PNAS nexus 4:pgaf147 PubMed40386679

2025

Interferon-dependent R-loop induction by Zika virus contributes to growth attenuation.

Applications

Unspecified application

Species

Unspecified reactive species

Yijing Zhao,Anna D Metzler,Yangping Li,Paola Urlich,Yilin Wang,David M Gilbert,Bing Yao,Hengli Tang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (AB234959)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (AB234959)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin gamma 1 antibody [EPR21200] - BSA and Azide free (AB234959)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise