AB244322

Anti-Langerin antibody

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Polyclonal Langerin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD207 aa 50-250.

대체 명칭 보기

CD207, CLEC4K, C-type lectin domain family 4 member K, Langerin

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody (AB244322)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody (AB244322)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Langerin with ab244322 at a 1/20 dilution in immunohistochemical analysis.

Low expression as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody (AB244322)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody (AB244322)

Formalin-fixed, paraffin-embedded human colon tissue stained for Langerin with ab244322 at a 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody (AB244322)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody (AB244322)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Langerin with ab244322 at a 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody (AB244322)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody (AB244322)

Formalin-fixed, paraffin-embedded human skin tissue stained for Langerin with ab244322 at a 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-Langerin antibody (AB244322)

WB

Unknown

Western blot - Anti-Langerin antibody (AB244322)

All lanes:

Western blot - Anti-Langerin antibody (ab244322) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

Langerin overexpression HEK-293T cell lysate

Predicted band size: 37 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

Recombinant Fragment Protein within Human CD207 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

style

left-column

대체품

Primary Antibodies

AB274894

Alexa Fluor® 555 Anti-Langerin antibody [EPR15863]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 555 Anti-Langerin antibody [EPR15863] (AB274894)

0
0

Primary Antibodies

AB225375

Alexa Fluor® 647 Anti-Langerin antibody [EPR15863]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Langerin antibody [EPR15863] (AB225375)

0
0

Primary Antibodies

AB232603

Anti-Langerin antibody [EPR15863] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation

Multiplex immunohistochemistry - Anti-Langerin antibody [EPR15863] - BSA and Azide free (AB232603)

0
0

Primary Antibodies

AB192027

Anti-Langerin antibody [EPR15863]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Langerin antibody [EPR15863] (AB192027)

5
1

보완 제품

Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

5
92

Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

5
75

Primary Antibodies

AB52866

Anti-alpha Tubulin antibody [EP1332Y] - Loading Control

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Trial Size

5
31

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

5
133

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Langerin also known as CD207 is a type II transmembrane protein involved in the immune response. It weighs approximately 40 kDa and is expressed mainly in Langerhans cells a specialized subset of dendritic cells found in the skin and mucosal tissues. Langerin functions as a C-type lectin receptor with the ability to recognize and internalize glycosylated antigens. This target facilitates the capture and presentation of antigens enhancing the immune surveillance process.

Biological function summary

Langerin supports Langerhans cells in the recognition and processing of pathogens. The protein forms homotrimers enabling it to bind specific mannose-rich glycan structures on pathogens such as viruses and bacteria. Through its endocytic properties Langerin internalizes these antigens into Birbeck granules unique organelles in Langerhans cells promoting antigen processing and presentation to T cells therefore initiating immune responses.

Pathways

Langerin is critical for antigen uptake and processing via the lectin pathway of endocytosis. It interacts with other dendritic cell receptors and influences signaling pathways that enhance immune activation such as the Syk and Raf-1 kinase pathways. Langerin’s ability to internalize antigens connects it with proteins like DC-SIGN and MR which share roles in pathogen recognition and immune modulation.

Langerin plays a significant role in conditions like Langerhans Cell Histiocytosis (LCH) and HIV. In LCH abnormal proliferation of Langerhans cells occurs where Langerin expression helps in diagnosing and characterizing LCH lesions. In the context of HIV Langerin interacts with viral particles reducing their ability to infect immune cells. This protein also relates to other immune receptors like CD40 and CD86 which regulate immune responses in associated disorders.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. Major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. Protects against human immunodeficiency virus-1 (HIV-1) infection. Binds to high-mannose structures present on the envelope glycoprotein which is followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation.

See full target information CD207

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of integrative neuroscience 20:595-603 PubMed34645092

2021

The impact of CRMP4 SUMOylation on the Cav1.2 interaction, neurite outgrowth and thermal pain sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Shangdao Lai,Meiyuan Pan,Hongxing Liao,Jiayu Chen,Yuqing Jiang,Yalan Li

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com