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AB316366

Anti-LAP2 alpha antibody [3A3]

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Mouse Monoclonal LAP2 alpha antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TMPO conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

LAP2, TMPO, Thymopoietin isoform alpha, Thymopoietin-related peptide isoform alpha, TP alpha, TPRP isoform alpha

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha Antibody [3A3] (AB316366)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha Antibody [3A3] (AB316366)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab316366 (red).

Western blot - Anti-LAP2 alpha Antibody [3A3] (AB316366)
  • WB

Supplier Data

Western blot - Anti-LAP2 alpha Antibody [3A3] (AB316366)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were boiled in Laemmli buffer and the proteins were separated by 8% SDS-PAGE and blotted onto nitrocellulose membrane. The membrane was blocked in 3% NFDM in PBS-T for 1h at RT and incubated at 4°C o/n with ab316366 at the indicated dilution in 0.5% NFDM/PBS-T. After incubation with anti-mouse Fc-HRP coupled secondary antibody for 1h at RT, ECL was performed with the Western Lightning reagents. A 30 second exposure is shown.

All lanes:

Western blot - Anti-LAP2 alpha Antibody [3A3] (ab316366) at 1 µg/mL

All lanes:

HeLa cell lysate

Secondary

All lanes:

anti-mouse Fc-HRP coupled secondary antibody

true

Exposure time: 30s

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

3A3

Isotype

IgG1

Light chain 타입

kappa

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TMPO conjugated to Keyhole Limpet Haemocyanin.

P42166

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보완 제품

Primary Antibodies

AB133741

Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)

  • 4
  • 15
Primary Antibodies

AB15440

Anti-Nucleophosmin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nucleophosmin antibody (AB15440)

  • 5
  • 5
Primary Antibodies

AB197740

HRP Anti-Tubulin antibody [YL1/2] - Loading Control

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Tubulin antibody [YL1/2] - Loading Control (AB197740)

  • 0
  • 0
Primary Antibodies

AB166630

Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker

RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin+Fibrillarin-like protein 1 antibody [EPR10823(B)] - Nucleolar Marker (AB166630)

  • 5
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
보관 버퍼
Preservative: 0.1% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1 month
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Lamina-associated polypeptide 2 alpha (LAP2 alpha) also known as LAP2A is part of the LAP2 protein family. It has a molecular mass of approximately 76 kDa. This protein connects with the nuclear lamina and is prominently expressed in the nucleus of most cell types. LAP2 alpha interacts with chromatin and its mechanical role involves regulating interactions between chromatin and nuclear structures.
Biological function summary

LAP2 alpha contributes to processes like nuclear stability and chromatin organization. It is involved in the formation of a complex with lamin A/C facilitating the association of this nuclear lamina protein. This interaction plays a significant role in dynamically organizing the nuclear architecture throughout the cell cycle influencing cell differentiation and proliferation.

Pathways

The interaction of LAP2 alpha with lamin A/C places it in the context of the nuclear envelope dynamics. Key pathways include the regulation of genome stability and DNA repair pathways. The interaction with lamin A/C influences how cells manage genome maintenance making LAP2 alpha important for cellular homeostasis.

LAP2 alpha shows relevance to muscular dystrophy and progeria. LAP2 alpha’s interaction with lamin A/C is important because mutations in lamin A/C are known to cause Emery-Dreifuss muscular dystrophy. Additionally LAP2 alpha's role in nuclear architecture is significant in the context of Hutchinson-Gilford progeria syndrome where abnormalities in nuclear shape are evident. In these diseases LAP2 alpha's interaction or malfunction can lead to or exacerbate pathology.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Plays an important role, together with LMNA, in the nuclear anchorage of RB1.. TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.
See full target information TMPO
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Product promise

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