AB242376

Anti-LAPTM4B antibody [CL5289]

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Mouse Monoclonal LAPTM4B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LAPTM4B aa 200-250.

대체 명칭 보기

PSEC0001, LAPTM4B, Lysosomal-associated transmembrane protein 4B, Lysosome-associated transmembrane protein 4-beta

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-LAPTM4B antibody [CL5289] (AB242376)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAPTM4B antibody [CL5289] (AB242376)

Immunocytochemistry/ Immunofluorescence anaysis of A431 cells labeling LAPTM4B with ab242376 at 2-10 μg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in vesicles and the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (AB242376)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (AB242376)

Paraffin-embedded human kidney tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (AB242376)

Paraffin-embedded human heart muscle tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (AB242376)

Paraffin-embedded human pancreas tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (AB242376)

Paraffin-embedded human cerebral cortex tissue stained for LAPTM4B using ab242376 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-LAPTM4B antibody [CL5289] (AB242376)

All lanes:

Western blot - Anti-LAPTM4B antibody [CL5289] (ab242376) at 1 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 41 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL5289

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LAPTM4B aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

Q86VI4

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LAPTM4B also known as Lysosomal Protein Transmembrane 4 Beta is a membrane protein involved in endolysosomal trafficking. This protein has a molecular mass of around 27 kDa. LAPTM4B is highly expressed in liver kidney and placenta tissue with notable expression in several types of solid tumors. Its location in endosomes and lysosomes suggests a role in cellular cargo sorting and transport processes.

Biological function summary

LAPTM4B is essential for regulating lysosomal function and intracellular signaling. This protein forms part of a complex with other lysosomal associated proteins and is involved in the regulation of lysosome positioning and signaling pathways. By modulating lysosome function LAPTM4B affects cellular processes like growth proliferation and apoptosis playing an important role in cell survival and homeostasis.

Pathways

This protein participates in the PI3K/Akt and MAPK signaling pathways which are critical for cell growth and survival. LAPTM4B interacts with proteins such as EGFR (Epidermal Growth Factor Receptor) and mTOR (mammalian target of rapamycin) which facilitate its role in these pathways. Its involvement in these pathways contributes to its regulatory function in cell survival and metabolism.

Researchers link LAPTM4B to cancer and osteoporosis. Overexpression of LAPTM4B has been observed in various types of cancers including liver and breast cancer where it may contribute to tumor growth and metastasis. In osteoporosis LAPTM4B interacts with proteins such as Cathepsin K which are involved in bone resorption suggesting its potential role in bone metabolism and health.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for optimal lysosomal function (PubMed : 21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed : 25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed : 25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed : 26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed : 26280656).

See full target information LAPTM4B

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Food science and biotechnology 34:743-755 PubMed39958166

2025

Long-term Pu-erh tea alleviates inflammatory bowel disease via the regulation of intestinal microbiota and maintaining the intestinal mucosal barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Bi-Yun Feng,Pei-Lu Chen,Ling Yan,Wei-Feng Huang,Cheng-Fu Li,Li-Tao Yi,Guang-Hui Xu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAPTM4B antibody [CL5289]

Immunocytochemistry/ Immunofluorescence - Anti-LAPTM4B antibody [CL5289] (AB242376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (AB242376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (AB242376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (AB242376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAPTM4B antibody [CL5289] (AB242376)

Western blot - Anti-LAPTM4B antibody [CL5289] (AB242376)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise