JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236954

Anti-LAS1L antibody

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Polyclonal LAS1L antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LAS1L aa 450 to C-terminus.

대체 명칭 보기

MSTP060, LAS1L, Ribosomal biogenesis protein LAS1L, Endoribonuclease LAS1L, Protein LAS1 homolog

1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-LAS1L antibody (AB236954)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAS1L antibody (AB236954)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for LAS1L (green) using ab236954 at 1/266 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated goat anti-rabbit IgG (H+L). Counterstained with DAPI (blue).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with primary antibody overnight at 4°C.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human LAS1L aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4W2

style
left-column

대체품

Primary Antibodies

AB140656

Anti-LAS1L antibody [EPR8988(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-LAS1L antibody [EPR8988(B)] (AB140656)

  • 0
  • 0

보완 제품

Primary Antibodies

AB75927

Anti-SSB antibody [mAbcam75927]

BOND RX™ Validated

Flow Cytometry (Intracellular) - Anti-SSB antibody [mAbcam75927] (AB75927)

  • 5
  • 4
Primary Antibodies

AB137869

Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosine Hydroxylase antibody [EP1532Y] - Neuronal Marker (AB137869)

  • 5
  • 9
Primary Antibodies

AB69522

Anti-TEX101 antibody

  • 0
  • 0
Primary Antibodies

AB170905

Anti-Tyrosinase antibody [EPR10141]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tyrosinase antibody [EPR10141] (AB170905)

  • 5
  • 2
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LAS1L also known as LAS1-like protein is a nucleolar protein with a molecular mass of approximately 80 kDa. It is expressed widely across human tissues with prominent presence in actively dividing cells. Mechanically LAS1L assists in the processing and maturation of pre-ribosomal RNA (rRNA) playing a critical role in ribosome biogenesis. This protein functions as part of a multi-protein complex ensuring precise assembly and function of ribosomes.
Biological function summary

LAS1L serves significant functions in the nucleolus within cell nucleuses. As a component of a larger complex called the pre-ribosome LAS1L coordinates with other proteins to facilitate the cleavage of pre-rRNA into smaller ribosomal RNA units. This activity is essential for producing functional ribosomal units which are important for protein synthesis. Its interaction with proteins such as GTPases and endonucleases is important for maintaining ribosome assembly fidelity.

Pathways

LAS1L operates within the ribosome biogenesis pathway closely interacting with proteins like PELP1 and NOL9. It plays a role in the mTOR signaling pathway impacting cell growth and proliferation. The protein also helps regulate downstream processes critical for the final assembly of ribosomal subunits integrating cellular nutrient signals with ribosome production.

LAS1L shows connections to various cancers and ribosomopathies. Alterations in LAS1L expression or function can lead to disrupted ribosomal biogenesis contributing to cancer pathogenesis due to uncontrolled cell division. Moreover LAS1L is linked with Diamond-Blackfan Anemia a disorder of ribosome production. Related proteins like NPM1 and RPL11 are involved in this condition highlighting the interplay between LAS1L and ribosome function-related diseases.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA (PubMed : 20647540). Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed : 22872859). Required for the efficient pre-rRNA processing at both ends of internal transcribed spacer 2 (ITS2) (PubMed : 22083961).
See full target information LAS1L
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology reports 41:3347-3354 PubMed31002350

2019

Upregulated histamine receptor H3 promotes tumor growth and metastasis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Deliang Yu,Jing Zhao,Yuhong Wang,Jing Hu,Qinchuan Zhao,Jibin Li,Jianjun Zhu
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com