AB115314

Anti-Lass5 antibody

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Rabbit Polyclonal Lass5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CERS5.

대체 명칭 보기

LASS5, CERS5, Ceramide synthase 5, CerS5, LAG1 longevity assurance homolog 5, Sphingoid base N-palmitoyltransferase CERS5, Sphingosine N-acyltransferase CERS5

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lass5 antibody (AB115314)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lass5 antibody (AB115314)

ab115314 at 5ug/ml staining Lass5 in Human small intestine tissue by immunohistochemistry (FFPE). After primary incubation slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Unknown

Western blot - Anti-Lass5 antibody (AB115314)

All lanes:

Western blot - Anti-Lass5 antibody (ab115314) at 1 µg/mL

Lane 1:

SK-N-SH cell lysate

Lane 2:

SK-N-SH cell lysate with blocking peptide

Predicted band size: 46 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CERS5. The exact immunogen used to generate this antibody is proprietary information.

Q8N5B7

Specificity

Multiple isoforms of Lass5 are known to exist. This antibody may cross-react with the highly homologous Lass6.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Lass5 also known as ceramide synthase 5 (CerS5) plays a critical role in ceramide production by catalyzing the condensation of sphinganine or sphingosine with palmitoyl-CoA. It has a molecular mass of approximately 42 kDa. Lass5 is primarily expressed in the liver heart and skeletal muscle and it is found in the endoplasmic reticulum membrane. This enzyme displays a preference for producing C16-ceramide due to its palmitoyl-CoA specificity.

Biological function summary

Lass5 contributes significantly to the regulation of cell proliferation apoptosis and response to stress. Lass5 forms part of a larger complex involved in sphingolipid metabolism which includes other ceramide synthases such as CerS2 and CerS4. By controlling ceramide levels Lass5 can influence cellular and physiological functions like autophagy and cell cycle arrest.

Pathways

Lass5 is heavily involved in the sphingolipid signaling pathway and the endoplasmic reticulum stress response pathway. These pathways are important for cell survival and homeostasis. Lass5 interacts with key proteins such as Bcl-2 family members which are critical for apoptosis regulation. It works together with other ceramide synthases to maintain cellular homeostasis and mediate various biological processes impacting cell function and survival.

Lass5 has been linked to cancer and neurodegenerative diseases. Overexpression or dysregulation of Lass5 can result in abnormal ceramide accumulation contributing to diseases like hepatocellular carcinoma. It may also play a role in the progression of neurodegenerative disorders such as Alzheimer's disease possibly through interactions with proteins like amyloid precursor protein (APP) in the neuronal context. Understanding Lass5's influence in these conditions can help in developing therapeutic strategies that target its function or regulation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward palmitoyl-CoA (hexadecanoyl-CoA; C16 : 0-CoA) (PubMed : 16951403, PubMed : 18541923, PubMed : 22144673, PubMed : 22661289, PubMed : 23530041, PubMed : 26887952, PubMed : 29632068, PubMed : 31916624). Can use other acyl donors, but with less efficiency (By similarity). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively (PubMed : 31916624). Plays a role in de novo ceramide synthesis and surfactant homeostasis in pulmonary epithelia (By similarity).

See full target information CERS5

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS pathogens 10:e1004412 PubMed25254972

2014

Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection.

Applications

Unspecified application

Species

Unspecified reactive species

Myung-Shin Lee,Tiffany Jones,Dae-Yong Song,Jae-Hyuk Jang,Jae U Jung,Shou-Jiang Gao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lass5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lass5 antibody (AB115314)

Western blot - Anti-Lass5 antibody (AB115314)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise