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AB325160

Anti-Lassa Fever virus GP1 antibody [HA6]

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Mouse Recombinant Monoclonal SHMT1 antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Lassa virus GA391, Recombinant full length protein - Lassa virus GA391, Lassa virus GA391 samples.
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Indirect ELISA - Anti-Lassa Fever virus GP1 antibody [HA6] (AB325160)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Lassa Fever virus GP1 antibody [HA6] (AB325160)

Indirect ELISA analysis of ab325160 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at 1 : 1000 dilution dilution.

Antigen : Lassa Fever Virus GP1 Protein;Lassa Fever Virus GP2 Protein.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Lassa Fever virus GP1 antibody [HA6] (AB325160)
  • WB

Lab

Western blot - Anti-Lassa Fever virus GP1 antibody [HA6] (AB325160)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Lassa Fever virus GP1 antibody [HA6] (ab325160) at 1/1000 dilution

Lane 1:

FC-tagged Lassa Fever Virus GP1 (strain GA391) recombinant protein at 10 ng

Lane 2:

FC-tagged Lassa Fever Virus GP2 (strain GA391) recombinant protein at 10 ng

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 75 kDa

false

Exposure time: 37s

Western blot - Anti-Lassa Fever virus GP1 antibody [HA6] (AB325160)
  • WB

Supplier Data

Western blot - Anti-Lassa Fever virus GP1 antibody [HA6] (AB325160)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1 : 1000 dilution.

All lanes:

Western blot - Anti-Lassa Fever virus GP1 antibody [HA6] (ab325160) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with Lassa virus (strain GA391) Glycoprotein G1(GP1) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with Lassa virus (strain GA391) Glycoprotein G2(GP2) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T transfected with Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) Glycoprotein G1(GP1) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 5:

293T transfected with Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) Glycoprotein G2(GP2) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 25 kDa,36 kDa

false

Exposure time: 26s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

HA6

Isotype

IgG1

Carrier free

No

Reacts with

Lassa virus GA391

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody does cross-react with Lassa virus (strain Mouse/Sierra Leone/Josiah/1976&#41; overexpressed Glycoprotein G1 (GP1&#41;.</p>

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보완 제품

Proteins & Peptides

AB256441

Recombinant Lassa Fever Virus GP1 glycoprotein (Fc Chimera)

SDS-PAGE - Recombinant Lassa Fever Virus GP1 glycoprotein (Fc Chimera) (AB256441)

  • 0
  • 0
Proteins & Peptides

AB48949

Recombinant SARS E protein (Coronavirus) (6x His tag C-Terminus)

  • 0
  • 0
Proteins & Peptides

AB236940

Recombinant Yersinia pestis F1 antigen protein (His tag)

SDS-PAGE - Recombinant Yersinia pestis F1 antigen protein (His tag) (AB236940)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Lassa virus GA391": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "<p></p>" }, "Recombinant full length protein - Lassa virus GA391": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Transfected cell lysate - Lassa virus GA391": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Stable signal peptide. Functions as a cleaved signal peptide that is retained as the third component of the GP complex (GP-C). Helps to stabilize the spike complex in its native conformation. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of G1 and G2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.. Glycoprotein G1. Forms the virion spikes together with glycoprotein G2. The glycoprotein spike trimers are connected to the underlying matrix. Interacts with the host receptor. Mediates virus attachment to the host primary receptor alpha-dystroglycan DAG1 (alpha-DG) at the cell surface. This attachment induces virion internalization apparently through macropinocytosis. Following endocytosis, there is a pH-dependent switch from binding DAG1 to the host lysosomal receptor LAMP1. This latter binding triggers the dissociation of GP1, exposing the fusion subunit, GP2, such that fusion can occur. Down-modulates host DAG1.. Glycoprotein G2. Forms the virion spikes together with glycoprotein G1. The glycoprotein spike trimers are connected to the underlying matrix. Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced by acidification.
See full target information GPC

대체 명칭 보기

GP-C, GPC, Pre-glycoprotein polyprotein GP complex, Pre-GP-C
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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