AB4476

Anti-LAT (phospho Y132) antibody

Name: Anti-LAT (phospho Y132) antibody
Brand: Abcam Limited
SKU: AB4476
Rating: 3 (1 reviews)

(1 리뷰)

(17 제품이 사용된 논문 )

Rabbit Polyclonal LAT phospho Y132 antibody. Suitable for WB and reacts with Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human LAT pY132.

1 이미지

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Western blot - Anti-LAT (phospho Y132) antibody (AB4476)

Peptide Competition
Extracts prepared from Jurkat E6.1 cells were left unstimulated (1) or stimulated (2-5), resolved by SDS-PAGE on a 10% polyacrylamide gel, and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.35 μg/ml phospho LAT (Tyr 132) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with : no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to phospho LAT (Tyr 132) completely blocks the antibody signal, thereby demonstrating the specificity of the antibody, and stimulation-induced tyrosine phosphorylation of phospho LAT (Tyr 132).

All lanes:

Western blot - Anti-LAT (phospho Y132) antibody (ab4476)

Predicted band size: 28 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human LAT pY132. The exact immunogen used to generate this antibody is proprietary information.

O43561

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated LAT. The final product is generated byaffinity chromatography using an LAT-derived peptide that is phosphorylated at tyrosine 132.

보관 버퍼

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LAT or Linker for Activation of T cells also known as LAT protein is a transmembrane adaptor protein with an approximate molecular mass of 36-38 kDa. This protein is expressed predominantly on T cells and other cells of the lymphoid lineage. LAT is localized to the plasma membrane and contains multiple tyrosine residues which are critical for its function in signal transduction. Its phosphorylation leads to binding of various signaling molecules enabling signal propagation.

Biological function summary

LAT plays an important role in the immune response by facilitating the assembly of signaling complexes important for T cell activation. Upon antigen stimulation LAT becomes phosphorylated mainly at tyrosine residues allowing proteins like Grb2 and PLCγ1 to form a functional signalosome complex. This complex formation is necessary for downstream signaling events that contribute to T cell proliferation differentiation and cytokine production thereby maintaining immune homeostasis and facilitating adaptive immune responses.

Pathways

LAT operates primarily within the T cell receptor (TCR) signaling pathway. The phosphorylation of LAT by ZAP-70 another important signaling protein enables its role as a scaffold bringing together other molecules for signal transduction. It is closely related to proteins like SLP-76 and GADS both of which are essential for the full activation of T cells. These interactions link LAT to broader signaling cascades such as the MAPK and calcium signaling pathways which are essential for mounting an immune response.

LAT dysfunction is linked to both autoimmune diseases and immunodeficiencies. Defective LAT expression or function can lead to conditions like severe combined immunodeficiency (SCID) due to impaired T cell signaling. Additionally abnormal LAT activity may contribute to autoimmune disorders like systemic lupus erythematosus (SLE) where an overactive immune response is present. Proteins such as LAT can interact with others involved in these conditions including PLCγ1 highlighting its central role in both healthy and diseased states of immune regulation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development (PubMed : 23514740, PubMed : 25907557). Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

See full target information LAT pY132

대체 명칭 보기

Linker for activation of T-cells family member 1, 36 kDa phosphotyrosine adapter protein, p36-38, pp36, LAT

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제품이 사용된 논문 (17)

Recent publications for all applications. Explore the 전체 목록 and refine your search

eLife 12: PubMed37796108

2023

Allosteric inhibition of the T cell receptor by a designed membrane ligand.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Ye,Shumpei Morita,Justin J Chang,Patrick M Buckley,Kiera B Wilhelm,Daniel DiMaio,Jay T Groves,Francisco N Barrera

Theranostics 12:3316-3328 PubMed35547746

2022

NAD-boosting molecules suppress mast cell degranulation and anaphylactic responses in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Woo Kim,Ga-Hee Ryoo,Hyun-Young Jang,So-Young Rah,Dong Hyun Lee,Do-Kyun Kim,Eun Ju Bae,Byung-Hyun Park

Frontiers in immunology 12:656366 PubMed34149695

2021

Identification and Characterization of the Amphioxus Lck and Its Associated Tyrosine Phosphorylation-Dependent Inhibitory LRR Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Jiatao Zhou,Zhihui Xiao,Yanli Zhan,Xuemei Qu,Sisi Mou,Chong Deng,Tianxiang Zhang,Xin Lan,Shengfeng Huang,Yingqiu Li

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:8959-8974 PubMed32469452

2020

PTPN22 interacts with EB1 to regulate T-cell receptor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaonan Zhang,Yang Yu,Bin Bai,Tao Wang,Jiahui Zhao,Na Zhang,Yanjiao Zhao,Xipeng Wang,Bing Wang

Frontiers in immunology 10:314 PubMed30873169

2019

T Cell Receptor (TCR)-Induced PLC-γ1 Sumoylation via PIASxβ and PIAS3 SUMO E3 Ligases Regulates the Microcluster Assembly and Physiological Function of PLC-γ1.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Long Wang,Jia-Qi Liang,Bei-Ni Gong,Ji-Ji Xie,Yu-Ting Yi,Xin Lan,Yingqiu Li

Frontiers in immunology 9:115 PubMed29456532

2018

A Stretch of Negatively Charged Amino Acids of Linker for Activation of T-Cell Adaptor Has a Dual Role in T-Cell Antigen Receptor Intracellular Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mikel M Arbulo-Echevarria,Isaac Narbona-Sánchez,Cecilia M Fernandez-Ponce,Inmaculada Vico-Barranco,Mª Dolores Rueda-Ygueravide,Michael L Dustin,Arkadiusz Miazek,Mª Carmen Duran-Ruiz,Francisco García-Cózar,Enrique Aguado

Journal of cell science 130:1217-1223 PubMed28209780

2017

Microtubule-associated protein-4 controls nanovesicle dynamics and T cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Eugenio Bustos-Morán,Noelia Blas-Rus,Noa Beatriz Martin-Cófreces,Francisco Sánchez-Madrid

Nature communications 7:11389 PubMed27091106

2016

Aurora A drives early signalling and vesicle dynamics during T-cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Noelia Blas-Rus,Eugenio Bustos-Morán,Ignacio Pérez de Castro,Guillermo de Cárcer,Aldo Borroto,Emilio Camafeita,Inmaculada Jorge,Jesús Vázquez,Balbino Alarcón,Marcos Malumbres,Noa B Martín-Cófreces,Francisco Sánchez-Madrid

The Journal of experimental medicine 213:155-65 PubMed26783323

2016

A novel human autoimmune syndrome caused by combined hypomorphic and activating mutations in ZAP-70.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Y Chan,Divya Punwani,Theresa A Kadlecek,Morton J Cowan,Jean L Olson,Erin F Mathes,Uma Sunderam,Shu Man Fu,Rajgopal Srinivasan,John Kuriyan,Steven E Brenner,Arthur Weiss,Jennifer M Puck

European journal of immunology 44:3741-6 PubMed25251667

2014

NF-κB is crucial in proximal T-cell signaling for calcium influx and NFAT activation.

Applications

Unspecified application

Species

Unspecified reactive species

Crystina C Bronk,Sean Yoder,Emily L Hopewell,Shengyu Yang,Esteban Celis,Xue-Zhong Yu,Amer A Beg

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAT (phospho Y132) antibody

Western blot - Anti-LAT (phospho Y132) antibody (AB4476)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (17)

Product promise