AB167416

Anti-LDLC antibody [EPR10896]

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal LDLC antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

LDLC, COG2, Conserved oligomeric Golgi complex subunit 2, COG complex subunit 2, Component of oligomeric Golgi complex 2, Low density lipoprotein receptor defect C-complementing protein

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LDLC with ab167416 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling LDLC with ab167416 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-LDLC antibody [EPR10896] (AB167416)

All lanes:

Western blot - Anti-LDLC antibody [EPR10896] (ab167416) at 1/1000 dilution

Lane 1:

Fetal lung tissue lysate at 10 µg

Lane 2:

Fetal brain tissue lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 83 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10896

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LDLC also known as Low-Density Lipoprotein Cholesterol is a significant component of the plasma lipid transport system. It plays a mechanical role in transporting cholesterol to peripheral tissues. The molecular mass of LDLC varies depending on the size of the lipoprotein particles typically ranging from 2000 to 3000 kDa. LDLC prominently circulates in the blood and is widely expressed throughout the body particularly in liver and intestines where it is synthesized and secreted.

Biological function summary

LDLC serves as the primary cholesterol carrier and is essential for cellular membrane synthesis and maintenance. It forms a complex with apolipoproteins such as ApoB-100 which facilitate interaction with LDL receptors on cell surfaces. This interaction enables the uptake of cholesterol for use in intracellular processes. LDL particles derive from very low-density lipoproteins (VLDL) following triglyceride depletion emphasizing their role in lipid metabolism.

Pathways

The cholesterol transport and lipid metabolism pathways involve LDLC as a core component. One important pathway is the mevalonate pathway where it links to biosynthesis of cholesterol steroids and isoprenoids. The protein SREBP (Sterol Regulatory Element Binding Protein) regulates the expression of LDL receptors impacting cholesterol uptake efficiency. Another relevant pathway is the reverse cholesterol transport pathway involving ApoA-I where excess cholesterol from tissues returns to the liver for excretion.

LDLC is strongly associated with cardiovascular diseases especially atherosclerosis. Elevated levels of LDLC contribute to plaque formation in arterial walls leading to heart diseases. Familial hypercholesterolemia a genetic disorder also links to increased LDLC levels due to defective LDL receptors caused by mutations in the LDLR gene. Apolipoprotein E (ApoE) polymorphisms further affect the clearance rate of LDLC influencing susceptibility to these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for normal Golgi morphology and function.

See full target information COG2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular and cellular biology 39: PubMed30988160

2019

Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex.

Applications

Unspecified application

Species

Unspecified reactive species

David McClellan,Mattie J Casey,Diana Bareyan,Helena Lucente,Christopher Ours,Matthew Velinder,Jason Singer,Mehraju Din Lone,Wenxiang Sun,Yunuen Coria,Clinton C Mason,Michael E Engel

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LDLC antibody [EPR10896]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LDLC antibody [EPR10896] (AB167416)

Western blot - Anti-LDLC antibody [EPR10896] (AB167416)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise