Anti-LEF1 Antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LEF1 Antibody (AB327174)

Immunofluorescent staining of human cell line U-251 MG using ab327174 at 0.25–2 µg/ml, showing localization to nucleoplasm & cytosol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 Antibody (AB327174)

Immunohistochemical staining of human oral mucosa using ab327174 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in subset of squamous epithelial cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEF1 Antibody (AB327174)

Immunohistochemical staining of human lymph node using ab327174 at 1 : 50–1 : 200 dilution, showing moderate to strong nuclear positivity in non - germinal center cells.

• ChIP

Supplier Data

ChIP - Anti-LEF1 Antibody (AB327174)

ChIP-Exo-Seq composite graph for ab327174 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-LEF1 Antibody (AB327174)

Western blot analysis in human cell lines SK-MEL-30 and A-549 using ab327174 at 0.04–0.4 µg/ml. Corresponding LEF1 RNA-seq data are presented for the same cell lines. Loading control : Anti-PPIB antibody.

All lanes:

Western blot - Anti-LEF1 Antibody (ab327174)

Lane 2:

Human cell lines SK-MEL-30

Lane 3:

Human cell lines A-549

Predicted band size: 44.2 kDa

false

• WB

Supplier Data

Western blot - Anti-LEF1 Antibody (AB327174)

Western blot analysis in control (vector only transfected HEK293T lysate) and LEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) using ab327174 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-LEF1 Antibody (ab327174)

Lane 2:

vector only transfected HEK293T lysate

Lane 3:

LEF1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Predicted band size: 44.2 kDa

false