AB104403

Anti-Leptin Receptor antibody

Name: Anti-Leptin Receptor antibody
Brand: Abcam Limited
SKU: AB104403
Rating: 3 (4 reviews)

BOND RX™ Validated
각 태그의 뜻

(4 리뷰들)

(16 제품이 사용된 논문 )

Rabbit Polyclonal Leptin Receptor antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 16 publications.

대체 명칭 보기

CD295, DB, OBR, LEPR, Leptin receptor, LEP-R, HuB219, OB receptor, OB-R

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin Receptor antibody (AB104403)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin Receptor antibody (AB104403)

IHC image of aLeptin Receptor staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104403, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-Leptin Receptor antibody (AB104403)

Leptin Receptor contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Leptin Receptor antibody (ab104403) at 1 µg/mL

All lanes:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 132 kDa

Observed band size: 150 kDa,40 kDa,49 kDa

true

Exposure time: 1min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Leptin Receptor commonly known as LEPR or OB-R is a protein that plays an important role in energy homeostasis and weight regulation. Mechanically it functions as a receptor for leptin the hormone predominantly produced by adipocytes. Leptin binding triggers activation of intracellular signaling pathways. The molecular weight of the Leptin Receptor can vary as it exists in several isoforms due to alternative splicing. It is prominently expressed in the hypothalamus and other tissues involved in energy regulation such as the liver and skeletal muscle.

Biological function summary

The Leptin Receptor acts as a gateway for leptin to exert its effects on the central nervous system and peripheral tissues. When leptin binds to LEPR it facilitates the formation of receptor complexes that engage downstream signaling molecules like STAT3 and MAPK. This results in regulation of appetite and energy expenditure. LEPR is not part of a complex with other receptors but it interacts with intracellular signaling proteins to mediate leptin's physiological effects.

Pathways

The Leptin Receptor is integral to the JAK-STAT signaling pathway and the MAPK pathway both of which are important in maintaining energy balance. LEPR activates JAK2 upon ligand binding leading to phosphorylation events that propagate the signal. It is closely associated with other proteins like SOCS3 which acts as a feedback inhibitor of leptin signaling playing a balancing role in the pathways mentioned.

The Leptin Receptor has significant associations with obesity and certain types of diabetes. LEPR mutations or deficiencies can lead to hyperphagia leading to severe obesity due to the disruption of normal leptin signaling. Additionally interactions between leptin and insulin signaling pathways can impact the development of Type 2 diabetes. In these conditions the Leptin Receptor often interacts with the insulin receptor highlighting its role in the complex network of metabolic regulation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Receptor for hormone LEP/leptin (Probable) (PubMed : 22405007). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (By similarity) (PubMed : 9537324). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed : 12504075, PubMed : 25060689, PubMed : 8805376). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production (By similarity).. Isoform A. May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3.. Isoform E. Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.

See full target information LEPR

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제품이 사용된 논문 (16)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomimetics (Basel, Switzerland) 9: PubMed39590245

2024

A Murine Model of Non-Wear-Particle-Induced Aseptic Loosening.

Applications

Unspecified application

Species

Unspecified reactive species

Vincentius Suhardi,Anastasia Oktarina,Yingzhen Niu,Branden Sosa,Julia Retzky,Matthew Greenblatt,Lionel Ivashkiv,Mathias Bostrom,Xu Yang

Nature biomedical engineering 8:1285-1307 PubMed39085645

2024

Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Vincentius Jeremy Suhardi,Anastasia Oktarina,Mohammed Hammad,Yingzhen Niu,Qingdian Li,Andrew Thomson,Juan Lopez,Jason McCormick,Ugur M Ayturk,Matthew B Greenblatt,Lionel B Ivashkiv,Mathias P G Bostrom,Xu Yang

JBMR plus 6:e10596 PubMed35309866

2022

A Subset of Osteosarcoma Bears Markers of CXCL12-Abundant Reticular Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Branden R Sosa,Ziqi Wang,John H Healey,Meera Hameed,Matthew B Greenblatt

Frontiers in veterinary science 8:625147 PubMed33644151

2021

Serum and Tissue Expression Levels of Leptin and Leptin Receptor Are Putative Markers of Specific Feline Mammary Carcinoma Subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia Gameiro,Catarina Nascimento,Ana Catarina Urbano,Jorge Correia,Fernando Ferreira

Evidence-based complementary and alternative medic 2020:2532081 PubMed33014100

2020

Effect of Qing'e Decoction on Leptin/Leptin Receptor and Bone Metabolism in Naturally Aging Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Sun,Yuanyuan Zhang,Zhenpu Wei,Zhiqiang Wang,Shiming Guo,Yanping Lin

Journal of orthopaedic translation 21:91-99 PubMed32110508

2020

Leptin receptor-expressing cells represent a distinct subpopulation of notochord-derived cells and are essential for disc homoeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Gao,Jinhua Yin,Xiaolong Xu,Jing Fan,Di Wang,Chao Zheng,Weiguang Lu,Pengzhen Cheng,Jicheng Sun,Dong Wang,Lu Li,Bo O Zhou,Liu Yang,Zhuojing Luo

PloS one 14:e0226010 PubMed31794592

2019

Influence of pre-pregnancy body mass index (p-BMI) and gestational weight gain (GWG) on DNA methylation and protein expression of obesogenic genes in umbilical vein.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Chavira-Suárez,Angélica Jazmín Ramírez-Mendieta,Sofía Martínez-Gutiérrez,Paola Zárate-Segura,Jorge Beltrán-Montoya,Nidia Carolina Espinosa-Maldonado,Juan Carlos de la Cerda-Ángeles,Felipe Vadillo-Ortega

PeerJ 7:e7624 PubMed31592340

2019

Expression of leptin and leptin receptors in colorectal cancer-an immunohistochemical study.

Applications

Unspecified application

Species

Unspecified reactive species

Saad M Al-Shibli,Norra Harun,Abdelkader E Ashour,Mohd Hanif B Mohd Kasmuri,Shaikh Mizan

Medical science monitor : international medical jo 25:3005-3013 PubMed31015393

2019

The Effect of the Leptin and Leptin Receptor Expression on the Efficacy of Neoadjuvant Chemotherapy in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Kong,Qian Dong,Hong Ji,Meixiang Sang,Yan Ding,Meng Zhao,Huichai Yang,Cuizhi Geng

Clinical epigenetics 11:20 PubMed30732639

2019

Maternal obesity influences expression and DNA methylation of the adiponectin and leptin systems in human third-trimester placenta.

Applications

Unspecified application

Species

Unspecified reactive species

Perrine Nogues,Esther Dos Santos,Hélène Jammes,Paul Berveiller,Lucie Arnould,François Vialard,Marie-Noëlle Dieudonné

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Leptin Receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Leptin Receptor antibody (AB104403)

Western blot - Anti-Leptin Receptor antibody (AB104403)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (16)

Product promise