Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (AB237031)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217572).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (AB237031)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cell line labeling LGALS3BP with ab217572 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217572).

• WB

Supplier Data

Western blot - Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (AB237031)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1 and 2 : 3 minutes; Lane 3 : 10 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID : 21526146, PMID : 21094132).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217572).

All lanes:

Western blot - Anti-LGALS3BP antibody [EPR21757-33] (<a href='/ko/products/primary-antibodies/lgals3bp-antibody-epr21757-33-ab217572'>ab217572</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human heart lysate at 10 µg

Lane 3:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/2000 dilution

Predicted band size: 65 kDa

Observed band size: 70 kDa,97 kDa

false

• WB

Supplier Data

Western blot - Anti-LGALS3BP antibody [EPR21757-33] - BSA and Azide free (AB237031)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 15 seconds; Lanes 3 and 4 : 136 seconds; Lane 4 : 3 minutes; Lane 5 : 15 seconds; Lanes 6,7,8 and 9 : 103 seconds.

The molecular mass observed is consistent with what has been described in the literature (PMID : 21526146, PMID : 21094132).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217572).

All lanes:

Western blot - Anti-LGALS3BP antibody [EPR21757-33] (<a href='/ko/products/primary-antibodies/lgals3bp-antibody-epr21757-33-ab217572'>ab217572</a>) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Rat liver lysate at 10 µg

Lane 3:

Rat spleen lysate at 10 µg

Lane 4:

Mouse heart lysate at 10 µg

Lane 5:

Mouse kidney lysate at 10 µg

Lane 6:

C6 (Rat glial tumor glial cell) whole cell lysate at 10 µg

Lane 7:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 9:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 65 kDa

Observed band size: 70 kDa,97 kDa

false