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AB199335

Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal LGR5 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 2 publications.
3 이미지
Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)
  • WB

Unknown

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)

This data was developed using ab75850, the same antibody clone in a different buffer formulation.

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-LGR5 antibody [EPR3065Y] (<a href='/ko/products/primary-antibodies/lgr5-antibody-epr3065y-ab75850'>ab75850</a>) at 1/1000 dilution

All lanes:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)
  • WB

Supplier Data

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)

This data was developed using ab75850, the same antibody clone in a different buffer formulation.

We are unsure about the identity of the additional band.

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-LGR5 antibody [EPR3065Y] (<a href='/ko/products/primary-antibodies/lgr5-antibody-epr3065y-ab75850'>ab75850</a>) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle lysate at 20 µg

Lane 2:

Rat skeletal muscle lysate at 20 µg

Lane 3:

Mouse brain lysate at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)
  • WB

Unknown

Western blot - Anti-LGR5 antibody [EPR3065Y] - BSA and Azide free (AB199335)

This data was developed using ab75850, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LGR5 antibody [EPR3065Y] (<a href='/ko/products/primary-antibodies/lgr5-antibody-epr3065y-ab75850'>ab75850</a>) at 1/1000 dilution

All lanes:

Fetal skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3065Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB261734

Anti-LGR5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody (AB261734)

  • 0
  • 0
Primary Antibodies

AB219107

Anti-LGR5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody (AB219107)

  • 4
  • 6

보완 제품

Sample Prep & Detection Kits

AB269899

PE/Texas Red Conjugation Kit - Lightning-Link®

Schematic Diagram - PE/Texas Red Conjugation Kit - Lightning-Link® (AB269899)

  • 0
  • 0
Sample Prep & Detection Kits

AB270228

Europium-Streptavidin

Functional Studies - Europium-Streptavidin (AB270228)

  • 5
  • 1
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

KD Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
Primary Antibodies

AB185966

Anti-SOX9 antibody [EPR14335-78]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (AB185966)

  • 5
  • 32
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

ab199335 is the carrier-free version of ab75850.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LGR5 also known as leucine-rich repeat-containing G-protein coupled receptor 5 is a transmembrane receptor primarily involved in the regulation of stem cell function. With a molecular mass of approximately 99 kDa LGR5 is highly expressed in intestinal crypts hair follicles and other tissues with proliferative capacity. This receptor functions through the activation of downstream signaling pathways upon binding with its ligand R-spondin. Often referred to as GPR49 LGR5 plays an essential role in embryonic development and adult tissue homeostasis.
Biological function summary

LGR5 marks adult stem cells in several rapidly self-renewing tissues. It forms part of a receptor complex that enhances Wnt signaling important for maintaining stemness and controlling cell fate. LGR5's activity ensures the self-renewal and differentiation balance within stem cell niches. The protein acts as a reliable stem cell marker in both intestinal and hair follicle stem cells facilitating their identification and isolation for research or therapeutic purposes.

Pathways

LGR5 interacts significantly with the Wnt signaling pathway playing a critical role in its activation. It associates with the R-spondin and LRP5/6 pathway components to potentiate Wnt signals thereby influencing cellular functions like proliferation and differentiation. LGR5's role in this pathway connects it with other key proteins such as β-catenin and TCF which are important for transcriptional regulation driven by Wnt pathway activation.

LGR5 expression links with colorectal cancer and medulloblastoma. In colorectal cancer overexpression of LGR5 suggests uncontrolled Wnt signaling leading to excessive cell proliferation. This association highlights its potential as a target for cancer therapy. Moreover LGR5 interacts with proteins like APC in tumor suppression and TP53 whose mutations often accompany tumorigenesis. Understanding LGR5's function and regulation helps develop therapeutic strategies for related pathologies.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.
See full target information LGR5

대체 명칭 보기

GPR49, GPR67, LGR5, Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:7808 PubMed40841558

2025

Control of Golgi- V-ATPase through Sac1-dependent co-regulation of PI(4)P and cholesterol.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhou,Miesje M van der Stoel,Shreyas Kaptan,Haoran Li,Shiqian Li,Maarit Hölttä,Helena Vihinen,Eija Jokitalo,Christoph Thiele,Olli Pietiläinen,Shin Morioka,Junko Sasaki,Takehiko Sasaki,Ilpo Vattulainen,Elina Ikonen

Frontiers in oncology 12:805290 PubMed35299743

2022

Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weitao Zhang,Lu Kong,Hongbin Zhu,Decong Sun,Quanli Han,Bin Yan,Zhi Cui,Weiwei Zhang,Shurong Zhang,Xindan Kang,Guanghai Dai,Niansong Qian,Wenji Yan
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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