Anti-LIM Kinase 1 antibody [OTI3G3]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (AB119084)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lymphoma tissue using ab119084 at a 1/150 dilution. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIM Kinase 1 antibody [OTI3G3] (AB119084)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human endometrium adenocarcinoma tissue using ab119084 at a 1/150 dilution. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

• WB

Lab

Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (AB119084)

Western blot : Anti-LIMK1 antibody [OTI3G3] (ab119084) staining at 1/2000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab119084 was shown to bind specifically to LIMK1. A band was observed at 73 kDa in wild-type A549 cell lysates with no signal observed at this size in LIMK1 knockout cell line. To generate this image, wild-type and LIMK1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-LIM Kinase 1 antibody [OTI3G3] (ab119084) at 1/2000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

LIMK1 knockout A549 cell lysate at 20 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

LIMK1 knockout HAP1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Observed band size: 73 kDa

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• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LIM Kinase 1 antibody [OTI3G3] (AB119084)

Immunofluorescent staining of COS-7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1 using ab119084 at a dilution of 1/100.