AB251257

Anti-LIM2 antibody [EPR16947] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(0 출판물)

Rabbit Recombinant Monoclonal LIM2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat samples.

대체 명칭 보기

Lens fiber membrane intrinsic protein, MP18, MP19, MP20, LIM2

2 이미지

Lab

Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (AB251257)

This data was developed using ab198502, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LIM2 antibody [EPR16947] (<a href='/ko/products/primary-antibodies/lim2-antibody-epr16947-ab198502'>ab198502</a>) at 1/50000 dilution

Lane 1:

Mouse eyeball tissue lysate at 10 µg

Lane 2:

Mouse brain tissue lysate at 10 µg

Lane 3:

Mouse heart tissue lysate at 10 µg

Lane 4:

Mouse kidney tissue lysate at 10 µg

Lane 5:

Mouse spleen tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 20 kDa

true

Exposure time: 30s

Lab

Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (AB251257)

This data was developed using ab198502, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LIM2 antibody [EPR16947] (<a href='/ko/products/primary-antibodies/lim2-antibody-epr16947-ab198502'>ab198502</a>) at 1/5000 dilution

Lane 1:

Rat eyeball tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Lane 3:

Rat heart tissue lysate at 10 µg

Lane 4:

Rat kidney tissue lysate at 10 µg

Lane 5:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 20 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16947

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

제품 세부 정보

ab251257 is the carrier-free version of ab198502.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The LIM2 protein also known as lens intrinsic membrane protein 2 is a lens-specific protein with a molecular mass of approximately 22 kDa. It is part of the eye lens fiber cells and is critical in maintaining lens transparency and structural integrity. LIM2 is highly expressed in the lens contributing to its function in the eye.

Biological function summary

LIM2 plays a significant role in lens development and homeostasis. It is part of the integral membrane proteins that influence lens fiber cell maturation and intercellular communication. LIM2 interacts with other cellular components supporting the structural arrangement necessary for proper lens function.

Pathways

LIM2 participates in cell adhesion and signal transduction pathways in the eye lens. These pathways are essential for maintaining cell shape transparency and communication within lens cells. LIM2 functions in concert with proteins like connexins which are also integral to cell-cell communication within the lens.

Mutations in the LIM2 gene have been linked to cataracts and other lens-related abnormalities. These conditions often result from disruptions in lens transparency and refractive properties that LIM2 helps maintain. The alpha crystallin and gamma crystallin proteins are related to LIM2 as their interactions affect lens clarity and structure contributing to cataract formation when dysfunctional.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Present in the thicker 16-17 nm junctions of mammalian lens fiber cells, where it may contribute to cell junctional organization. Acts as a receptor for calmodulin. May play an important role in both lens development and cataractogenesis.

See full target information LIM2

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LIM2 antibody [EPR16947] - BSA and Azide free

Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (AB251257)

Western blot - Anti-LIM2 antibody [EPR16947] - BSA and Azide free (AB251257)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise