Anti-Liver Arginase antibody [EPR19652] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] - BSA and Azide free (AB232932)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Liver Arginase with ab203490 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control : No staining on mouse kidney (PMID : 21975054). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203490).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] - BSA and Azide free (AB232932)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Liver Arginase with ab203490 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat liver (PMID : 20661013; PMID : 21975054). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230490).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody [EPR19652] - BSA and Azide free (AB232932)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine tissue labeling Liver Arginase with ab203490 at 1/100 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Negative staining on mouse small intestine (PMID : 21975054) is observed. Counter stained with DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203490).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Liver Arginase antibody [EPR19652] - BSA and Azide free (AB232932)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Liver Arginase with ab203490 at 1/100 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in the hepatic cells of mouse liver (PMID : 21975054) is observed. Counter stained with DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.

Perform heat-mediated antigen retrieval by using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203490).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR19652] - BSA and Azide free (AB232932)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Liver Arginase with ab203490 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse liver (PMID : 20661013; PMID : 21975054). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203490).

• WB

Lab

Western blot - Anti-Liver Arginase antibody [EPR19652] - BSA and Azide free (AB232932)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

The expression profile observed in RAW 264.7 is consistent with the literature (PMID : 28032600, PMID : 27166374, PMID : 9814991, PMID : 11080091).
Negative control : RAW 264.7 (PMID : 28032600, PMID : 27166374, PMID : 9814991, PMID : 11080091).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203490).

All lanes:

Western blot - Anti-Liver Arginase antibody [EPR19652] (<a href='/ko/products/primary-antibodies/liver-arginase-antibody-epr19652-ab203490'>ab203490</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 35 kDa

Observed band size: 37 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-Liver Arginase antibody [EPR19652] - BSA and Azide free (AB232932)

This data was developed using ab203490, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

Low/Negative sample : kidney, spleen (PMID : 21975054).

All lanes:

Western blot - Anti-Liver Arginase antibody [EPR19652] (<a href='/ko/products/primary-antibodies/liver-arginase-antibody-epr19652-ab203490'>ab203490</a>) at 1/5000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 20 µg

Lane 6:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 35 kDa,36 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-Liver Arginase antibody [EPR19652] - BSA and Azide free (AB232932)

This data was developed using ab203490, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid- femtogram range.

All lanes:

Western blot - Anti-Liver Arginase antibody [EPR19652] (<a href='/ko/products/primary-antibodies/liver-arginase-antibody-epr19652-ab203490'>ab203490</a>) at 1/5000 dilution

Lane 1:

Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 treated with 40 ng/ml IL-4 for 4 days, then treated with 40 ng/ml IL-10 for 4 days whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 35 kDa,36 kDa

true

Exposure time: 140s