AB63473

Anti-LKB1 (phospho S428) antibody

Name: Anti-LKB1 (phospho S428) antibody
Brand: Abcam Limited
SKU: AB63473
Rating: 4 (1 reviews)

(1 리뷰)

(13 제품이 사용된 논문 )

Rabbit Polyclonal LKB1 phospho S428 antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human STK11 pS428.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-LKB1 (phospho S428) antibody (AB63473)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LKB1 (phospho S428) antibody (AB63473)

ab63473 at 1/500 dilution staining LKB1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Unknown

Western blot - Anti-LKB1 (phospho S428) antibody (AB63473)

All lanes:

Western blot - Anti-LKB1 (phospho S428) antibody (ab63473) at 1/500 dilution

Lane 1:

mouse brain extracts at 30 µg

Lane 2:

mouse brain extracts at 30 µg with immunising peptide

Predicted band size: 48 kDa

Observed band size: 45 kDa,48 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human STK11 pS428. The exact immunogen used to generate this antibody is proprietary information.

Q15831

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab63473 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LKB1 also known as STK11 is a serine/threonine kinase with a molecular weight of approximately 48 kDa. This protein is widely expressed in various tissues including the liver pancreas and skeletal muscle. LKB1 plays an essential role in cellular energy homeostasis by phosphorylating and activating a group of at least 14 different proteins within the AMP-activated protein kinase (AMPK) family. The LKB1 protein acts as a master upstream kinase ensuring proper cellular energy balance and metabolism under conditions of nutrient stress.

Biological function summary

LKB1 is involved in controlling cell growth polarity and metabolism. It forms a complex with STRAD and MO25 which are necessary for its activation and function. By influencing these biological processes LKB1 regulates cell cycle arrest in response to stress signals and contributes to maintaining cellular integrity. The regulation of these processes positions LKB1 as an important factor in managing how cells respond to metabolic challenges.

Pathways

Its function integrates into major signaling networks like the AMPK and mTOR pathways. LKB1 activates AMPK which in turn affects mTOR signaling a pathway critical for cell growth and autophagy regulation. LKB1's interaction with AMPK links cellular energy levels to the control of biosynthetic pathways and cell cycle progression. The LKB1-AMPK-mTOR axis for instance highlights the protein's role in managing how cells balance growth-related activities and energy conservation.

LKB1 mutations or malfunctions are linked to Peutz-Jeghers syndrome and various forms of cancer such as lung cancer. Altered LKB1 activity affects its ability to suppress tumors primarily through its pathway interactions with AMPK and mTOR. Dysfunction in this pathway leads to unregulated cell growth and subsequently tumorigenesis. Understanding LKB1's role in these conditions aids in developing targeted therapies and diagnostic tools to manage associated risks and interventions effectively.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity : phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including : inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response : interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53 : translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed : 25329316).. Isoform 2. Has a role in spermiogenesis.

See full target information STK11 pS428

대체 명칭 보기

LKB1, PJS, STK11, Serine/threonine-protein kinase STK11, Liver kinase B1, Renal carcinoma antigen NY-REN-19, hLKB1

style

left-column

제품이 사용된 논문 (13)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in pharmacology 16:1524219 PubMed40635757

2025

Dexmedetomidine reduces acute lung injury caused by LPS through the SIRT3 signaling pathway .

Applications

Unspecified application

Species

Unspecified reactive species

Jian Chen,Yang Cai,Xiaochun Peng,Yuanling Xu,Liying Chen,Xinxin Pan,Yingying Sun

Heliyon 10:e36140 PubMed39253164

2024

GPRASP2 deficiency contributes to apoptosis in the spiral ganglion cells via the AMPK/DRP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Huang,Jing Cai,Yajie Lu,Tianming Wang,Shen Yue,Qinjun Wei,Jun Yao,Zhibin Chen,Xin Cao

EMBO reports 24:e54006 PubMed36416244

2022

Mitophagy bridges DNA sensing with metabolic adaption to expand lung cancer stem-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Liu,Shan Shan,Zixin Yuan,Fengying Wu,Ming Zheng,Ying Wang,Jun Gui,Wei Xu,Chunhong Wang,Tao Ren,Zhenke Wen

Bioengineered 13:13667-13679 PubMed35703318

2022

lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cansheng Zhu,Min Wang,Xianguan Yu,Xing Shui,Leile Tang,Zefeng Chen,Zhaojun Xiong

Bioengineered 13:12409-12420 PubMed35603707

2022

Wnt family member 1 (Wnt1) overexpression-induced M2 polarization of microglia alleviates inflammation-sensitized neonatal brain injuries.

Applications

Unspecified application

Species

Unspecified reactive species

Jinzhi Gao,Hao Xu,Zhihui Rong,Ling Chen

Frontiers in pharmacology 12:754976 PubMed34566665

2021

Ferulic Acid Ameliorates Hepatic Inflammation and Fibrotic Liver Injury by Inhibiting PTP1B Activity and Subsequent Promoting AMPK Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzhi Wu,Xiaoyong Xue,Guifang Fan,Yiqing Gu,Fei Zhou,Qi Zheng,Runping Liu,Yajing Li,Boning Ma,Shuo Li,Guangrui Huang,Lin Ma,Xiaojiaoyang Li

Cell biology international 45:305-319 PubMed33079432

2020

PSPH induces cell autophagy and promotes cell proliferation and invasion in the hepatocellular carcinoma cell line Huh7 via the AMPK/mTOR/ULK1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianli Zhang,Erhao Wang,Lei Zhang,Bo Zhou

Nature medicine 25:620-627 PubMed30833748

2019

Protective autophagy elicited by RAF→MEK→ERK inhibition suggests a treatment strategy for RAS-driven cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Conan G Kinsey,Soledad A Camolotto,Amelie M Boespflug,Katrin P Guillen,Mona Foth,Amanda Truong,Sophia S Schuman,Jill E Shea,Michael T Seipp,Jeffrey T Yap,Lance D Burrell,David H Lum,Jonathan R Whisenant,G Weldon Gilcrease,Courtney C Cavalieri,Kaitrin M Rehbein,Stephanie L Cutler,Kajsa E Affolter,Alana L Welm,Bryan E Welm,Courtney L Scaife,Eric L Snyder,Martin McMahon

Cardiovascular research 114:1738-1751 PubMed29800228

2018

PCSK9 expression in the ischaemic heart and its relationship to infarct size, cardiac function, and development of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Zufeng Ding,Xianwei Wang,Shijie Liu,Jiwani Shahanawaz,Sue Theus,Yubo Fan,Xiaoyan Deng,Sichang Zhou,Jawahar L Mehta

Molecular medicine reports 17:2837-2844 PubMed29257268

2017

Activation of the LKB1‑SIK1 signaling pathway inhibits the TGF‑β‑mediated epithelial‑mesenchymal transition and apoptosis resistance of ovarian carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Jianmei Zhang,Wenlan Yang

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LKB1 (phospho S428) antibody

Immunocytochemistry/ Immunofluorescence - Anti-LKB1 (phospho S428) antibody (AB63473)

Western blot - Anti-LKB1 (phospho S428) antibody (AB63473)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (13)

Product promise