AB32583

Anti-LMAN1 antibody

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Rabbit Polyclonal LMAN1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Rat Lman1 aa 150-200.

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

ab32583 labelling LMAN1 in HMVEC cells by Immunocytochemistry/Immunofluorescence.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

ab32583 labelling LMAN1 in A549 cells by Immunocytochemistry/Immunofluorescence.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

ab32583 labelling LMAN1 in p19 cells by Immunocytochemistry/Immunofluorescence.

Unknown

Western blot - Anti-LMAN1 antibody (AB32583)

All lanes:

Western blot - Anti-LMAN1 antibody (ab32583) at 1/1000 dilution

Lane 1:

Rat brain lysate

Lane 2:

Rat brain lysate with Immunising peptide

Predicted band size: 58 kDa

Observed band size: 57.5 kDa

false

Supplier Data

Western blot - Anti-LMAN1 antibody (AB32583)

All lanes:

Western blot - Anti-LMAN1 antibody (ab32583) at 1/2000 dilution

Lane 1:

HepG2 lysate at 30 µg

Lane 2:

HeLa lysate at 30 µg

Lane 3:

PANC-1 lysate at 30 µg

Lane 4:

MCF7 lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 58 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Rat Lman1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q62902

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LMAN1 also known as ERGIC-53 or lectin mannose-binding 1 is a protein involved in the transport and quality control of glycoproteins. It has a mass of approximately 53 kDa. LMAN1 primarily expresses in the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and is important for efficient cargo sorting and proper folding. It functions by binding to specific carbohydrate moieties on glycoproteins aiding their transport between the ER and Golgi apparatus.

Biological function summary

LMAN1 functions significantly within a protein complex known as the LMAN1-MCFD2 complex. It serves an essential role as a cargo receptor for glycoproteins particularly those with particular mannose-rich domains influencing their secretion and stability. This protein complex is integral in maintaining protein homeostasis and cellular physiology by ensuring the correct sorting and transport of glycoproteins from the ER to the Golgi.

Pathways

LMAN1 significantly participates in the secretory pathway and quality control mechanisms in the cell. It interacts with proteins such as MCFD2 to mediate the transport of specific glycoproteins like α1-antitrypsin through the secretory pathway. This pathway is important for the secretion of a variety of proteins and disruptions can lead to disorders associated with improper protein folding and trafficking.

Defects in LMAN1 can lead to bleeding disorders such as combined coagulation factor V and VIII deficiency. The protein's dysfunction impairs the transport of coagulation factors disrupting their secretion. Additionally proteins like MCFD2 work closely with LMAN1 in this context as mutations in either protein can cause a similar deficiency highlighting the significant role of LMAN1 in maintaining normal hemostatic processes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.

See full target information LMAN1

대체 명칭 보기

ERGIC53, F5F8D, LMAN1, Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

eLife 7: PubMed29334356

2018

p53 orchestrates DNA replication restart homeostasis by suppressing mutagenic RAD52 and POLθ pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sunetra Roy,Karl-Heinz Tomaszowski,Jessica W Luzwick,Soyoung Park,Jun Li,Maureen Murphy,Katharina Schlacher

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LMAN1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

Immunocytochemistry/ Immunofluorescence - Anti-LMAN1 antibody (AB32583)

Western blot - Anti-LMAN1 antibody (AB32583)

Western blot - Anti-LMAN1 antibody (AB32583)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise