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AB249151

Anti-LNK antibody [EPR7342] - BSA and Azide free

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Rabbit Recombinant Monoclonal LNK antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

LNK, SH2B3, SH2B adapter protein 3, Lymphocyte adapter protein, Lymphocyte-specific adapter protein Lnk, Signal transduction protein Lnk

1 이미지
Western blot - Anti-LNK antibody [EPR7342] - BSA and Azide free (AB249151)
  • WB

Unknown

Western blot - Anti-LNK antibody [EPR7342] - BSA and Azide free (AB249151)

This data was developed using ab154848, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LNK antibody [EPR7342] (<a href='/ko/products/primary-antibodies/lnk-antibody-epr7342-ab154848'>ab154848</a>) at 1/1000 dilution

Lane 1:

Ramos cell lysate at 10 µg

Lane 2:

MOLT4 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Predicted band size: 63 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7342

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB32049

Anti-p53 antibody [Y5]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 antibody [Y5] (AB32049)

  • 4
  • 5
Primary Antibodies

AB74115

Anti-Polycystin 1/PC1 antibody [7e12]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polycystin 1/PC1 antibody [7e12] (AB74115)

  • 3
  • 2
Primary Antibodies

AB108349

Anti-CDKN2A/p16INK4a antibody [EPR1473] - C-terminal

BOND RX™ Validated|20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDKN2A/p16INK4a antibody [EPR1473] - C-terminal (AB108349)

  • 4
  • 7
Primary Antibodies

AB7291

Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB7291)

  • 5
  • 92
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

ab249151 is the carrier-free version of ab154848.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LNK also known as SH2B3 is an adaptor protein about 70 kDa in mass. LNK contains an SH2 domain and is expressed mainly in hematopoietic cells including stem and progenitor cells. This protein inhibits signaling pathways by binding with receptors and blocking further transduction. It works by negatively regulating signals mediated by cytokine receptors and receptor tyrosine kinases.
Biological function summary

The LNK protein functions as a negative regulator in the hematopoietic system helping to control cell proliferation and immune response. LNK interacts with other signaling proteins as part of regulatory complexes often utilizing its SH2 domain to bind phosphorylated tyrosines. By doing so it inhibits the activity of various kinases involved in cell division ensuring proper signaling and growth control. This role is important to maintaining cellular homeostasis and preventing excessive cell proliferation.

Pathways

The LNK protein plays significant roles in the JAK-STAT and PI3K-AKT signaling pathways. In these pathways LNK interacts with signaling molecules like JAK2 and STAT5 to limit excessive signaling. This interaction is known to affect cellular responses to growth factors and cytokines impacting processes such as cell survival and proliferation. Through its activity in these pathways LNK helps ensure appropriate cellular responses to external stimuli.

LNK has connections with conditions like myeloproliferative neoplasms and hypertension. Mutations or aberrant expression of LNK can lead to dysregulated signaling that contributes to these diseases. In myeloproliferative neoplasms altered LNK function can lead to uncontrolled cell proliferation through interactions with proteins such as JAK2. In hypertension its role in regulating vascular tone and inflammation highlights its potential involvement in the pathogenesis of the disease.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Links T-cell receptor activation signal to phospholipase C-gamma-1, GRB2 and phosphatidylinositol 3-kinase.
See full target information SH2B3
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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