AB190255

Anti-LNPEP antibody - N-terminal

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Rabbit Polyclonal LNPEP antibody. N-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LNPEP aa 1-50.

대체 명칭 보기

OTASE, LNPEP, Leucyl-cystinyl aminopeptidase, Cystinyl aminopeptidase, Insulin-regulated membrane aminopeptidase, Insulin-responsive aminopeptidase, Oxytocinase, Placental leucine aminopeptidase, IRAP, OTase, P-LAP

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Supplier Data

Immunoprecipitation - Anti-LNPEP antibody - N-terminal (AB190255)

Detection of LNPEP by Western Blot of Immunoprecipitate.
ab190255 at 1 μg/ml labeling LNPEP in Jurkat whole cell lysate immunoprecipitated using ab190255 at 6 μg/reaction (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-LNPEP antibody - N-terminal (ab190255)

Predicted band size: 117 kDa

false

Supplier Data

Western blot - Anti-LNPEP antibody - N-terminal (AB190255)

All lanes:

Western blot - Anti-LNPEP antibody - N-terminal (ab190255) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 117 kDa

true

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human LNPEP aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UIQ6

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab190255 was affinity purified using an epitope specific to LNPEP immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LNPEP also called leucyl/cystinyl aminopeptidase or insulin-regulated membrane aminopeptidase (IRAP) serves as a zinc-dependent aminopeptidase. This protein cleaves N-terminal amino acids from peptide substrates functioning inside various tissues like the heart brain and placenta. The mass of LNPEP is approximately 140 kDa. It associates with the endosome compartment in cells and resides at the plasma membrane linking to vesicular transport processes.

Biological function summary

LNPEP plays a role in peptide hormone regulation and recognition involving itself in glucose homeostasis and blood pressure regulation. It joins part of complexes involved in peptide recycling and processing within endocytic vesicles. This protein regulates the degradation of peptide hormones like oxytocin and vasopressin indicating its function extends to neuropeptide modulation.

Pathways

The activity of LNPEP spans significant biological pathways such as the renin-angiotensin system where it modulates the levels of angiotensin IV impacting cardiovascular functions and influencing blood pressure regulation. It closely relates to proteins like insulin-responsive aminopeptidase and angiotensin IV receptor enhancing its regulatory role within these pathways.

LNPEP significantly links to hypertension and diabetes. The modulation of angiotensin IV by LNPEP directly impacts blood pressure associating its activity with the development and management of hypertension. Additionally LNPEP's impact on glucose uptake and insulin action connects it with diabetic conditions. It exhibits interactions with proteins such as the glucose transporter type 4 (GLUT4) in the context of diabetes highlighting its role in metabolic regulation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain.

See full target information LNPEP

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular medicine (Cambridge, Mass.) 31:199 PubMed40399808

2025

Metformin reverses 5-FU resistance induced by radiotherapy through mediating folate metabolism in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuxuan Wang,Yanyan Lin,Qianqian Zhao,Huanliang Chen,Shisuo Du,Zhaochong Zeng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LNPEP antibody - N-terminal

Immunoprecipitation - Anti-LNPEP antibody - N-terminal (AB190255)

Western blot - Anti-LNPEP antibody - N-terminal (AB190255)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise