Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (AB240345)

Immunohistochemical analysis of paraffin-embedded human uterus tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin. Used as a negative tissue control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (AB240345)

Immunocytochemistry/ Immunofluorescence analysis of A431 cell line labeling Loricrin with ab198994 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.1% Triton X-100. Alexa Fluor^® 488 Goat anti-Rabbit ab150077 used as the secondary at a 1/1000 dilution. Counterstained using ab7291 anti-Tubulin (mouse mAb) at a 1/1000 dilution and ab150120 Alexa Fluor^® 594 Goat anti-Mouse secondary at a 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (AB240345)

Immunohistochemical analysis of paraffin-embedded human skin tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Loricrin antibody [EPR7149(2)] - BSA and Azide free (AB240345)

Immunohistochemical analysis of paraffin-embedded rat skin tissue sections labeling Loricrin with ab198994 at a 1/500 dilution. Goat anti-rabbit IgG H&L (HRP) ab97051 used as the secondary at a 1/500 dilution. Counterstain hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198994).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.