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AB213598

Anti-LOXL2 antibody [EPR12733] - BSA and Azide free

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Rabbit Recombinant Monoclonal LOXL2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.
1 이미지
Western blot - Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (AB213598)
  • WB

Lab

Western blot - Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (AB213598)

This data was developed using the same antibody clone in a different buffer formulation (ab179810).

Lanes 1-4 : Merged signal (red and green). Green - ab179810 observed at 105 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab179810 Anti-LOXL2 antibody [EPR12733] - C-terminal was shown to specifically react with LOXL2 in wild-type HeLa cells. Loss of signal was observed when knockout HeLa cells were used. Wild-type and LOXL2 knockout samples were subjected to SDS-PAGE. ab179810 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (<a href='/ko/products/primary-antibodies/loxl2-antibody-epr12733-c-terminal-ab179810'>ab179810</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

LOXL2 knockout HeLa cell lysate at 20 µg

Lane 3:

HeLa treated with 0.5nM CoCl2 for 6 hours whole cell lysate at 20 µg

Lane 4:

Untreated HeLa cell lysate at 20 µg

Predicted band size: 87 kDa

Observed band size: 105 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (2)

  • Unconjugated

    Anti-LOXL2 antibody [EPR12733] - C-terminal

  • HRP

    HRP Anti-LOXL2 antibody [EPR12733] - C-terminal

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12733

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>Our lab has been unable to use this product successfully in IHC-P and so we cannot guarantee that it will work in this application. However, some customers have been successful with this antibody in IHC-P.</p>

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대체품

Primary Antibodies

AB96233

Anti-LOXL2 antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-LOXL2 antibody (AB96233)

  • 4
  • 9
Primary Antibodies

AB197779

Anti-LOXL2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOXL2 antibody (AB197779)

  • 0
  • 0
Primary Antibodies

AB140833

Anti-LOXL2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOXL2 antibody (AB140833)

  • 5
  • 3
Primary Antibodies

AB260046

Anti-LOXL2 antibody [EPR23035-105]

RabMAb|Recombinant

Immunoprecipitation - Anti-LOXL2 antibody [EPR23035-105] (AB260046)

  • 0
  • 0
Primary Antibodies

AB267495

Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (AB267495)

  • 0
  • 0
Primary Antibodies

AB133383

Anti-LOXL2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOXL2 antibody (AB133383)

  • 1
  • 1

보완 제품

Assay Kits

AB213808

Human LOXL2 ELISA Kit

Sandwich ELISA - Human LOXL2 ELISA Kit (AB213808)

  • 0
  • 0
Sample Prep & Detection Kits

AB269900

PE/Atto 594 Conjugation Kit - Lightning-Link®

Schematic Diagram - PE/Atto 594 Conjugation Kit - Lightning-Link® (AB269900)

  • 0
  • 0
Sample Prep & Detection Kits

AB269893

Latex Conjugation Kit - 400nm Red

Functional Studies - Latex Conjugation Kit - 400nm Red (AB269893)

  • 0
  • 0
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

KD Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB174316

Anti-LOX antibody [EPR4025]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (AB174316)

  • 4
  • 5
Primary Antibodies

AB319041

Anti-LOXL3 antibody [EPR28299-24]

KD Validated|20ul selling size|Recombinant|RabMAb

Western blot - Anti-LOXL3 antibody [EPR28299-24] (AB319041)

  • 0
  • 0
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

ab213598 is the carrier-free version of ab179810.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine) (PubMed : 27735137). Acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation (PubMed : 27735137). Shows no activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2) (PubMed : 27735137). Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription (PubMed : 25959397). LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2 (By similarity). Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin CDH1, probably by mediating deamination of histone H3 (PubMed : 16096638, PubMed : 24414204, PubMed : 27735137). During EMT, involved with SNAI1 in negatively regulating pericentromeric heterochromatin transcription (PubMed : 24239292). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (PubMed : 24239292). Interacts with the endoplasmic reticulum protein HSPA5 which activates the IRE1-XBP1 pathway of the unfolded protein response, leading to expression of several transcription factors involved in EMT and subsequent EMT induction (PubMed : 28332555). Involved in E-cadherin repression following hypoxia, a hallmark of EMT believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression (PubMed : 20026874). When secreted into the extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed : 20306300). Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding (PubMed : 21835952). Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation (By similarity).
See full target information LOXL2

대체 명칭 보기

Lysyl oxidase homolog 2, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, LOXL2
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Genes & development 33:747-762 PubMed31123067

2019

Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Pearson,Anne Loft,Prashant Rajbhandari,Judith Simcox,Sanghoon Lee,Peter Tontonoz,Susanne Mandrup,Claudio J Villanueva
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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