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AB267495

Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free

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Rabbit Recombinant Monoclonal LOXL2 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
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Immunoprecipitation - Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (AB267495)
  • IP

Unknown

Immunoprecipitation - Anti-LOXL2 antibody [EPR23035-105] - BSA and Azide free (AB267495)

LOXL2 was immunoprecipitated from 0.35 mg MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate with ab260046 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260046 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1 : MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate 10ug

Lane 2 : ab260046 IP in MDA-MB-435S whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab260046 in MDA-MB-435S whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

LOXL2 is a glycoprotein. Unprocessed LOXL2 (100kDa) and processed LOXL2(70kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 27770022).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260046).

All lanes:

Immunoprecipitation - Anti-LOXL2 antibody [EPR23035-105] (<a href='/ko/products/primary-antibodies/loxl2-antibody-epr23035-105-ab260046'>ab260046</a>)

Predicted band size: 87 kDa

Observed band size: 100 kDa,70 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23035-105

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB96233

Anti-LOXL2 antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-LOXL2 antibody (AB96233)

  • 4
  • 9
Primary Antibodies

AB202536

HRP Anti-LOXL2 antibody [EPR12733] - C-terminal

RabMAb|Recombinant

Western blot - HRP Anti-LOXL2 antibody [EPR12733] - C-terminal (AB202536)

  • 0
  • 0
Primary Antibodies

AB179810

Anti-LOXL2 antibody [EPR12733] - C-terminal

20ul selling size|RabMAb|Recombinant

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (AB179810)

  • 4
  • 6
Primary Antibodies

AB213598

Anti-LOXL2 antibody [EPR12733] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-LOXL2 antibody [EPR12733] - BSA and Azide free (AB213598)

  • 0
  • 0
Primary Antibodies

AB140833

Anti-LOXL2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOXL2 antibody (AB140833)

  • 5
  • 3

보완 제품

Assay Kits

AB213808

Human LOXL2 ELISA Kit

Sandwich ELISA - Human LOXL2 ELISA Kit (AB213808)

  • 0
  • 0
Primary Antibodies

AB174316

Anti-LOX antibody [EPR4025]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LOX antibody [EPR4025] (AB174316)

  • 4
  • 5
Assay Kits

AB275893

Human LOXL2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human LOXL2 ELISA Kit (AB275893)

  • 0
  • 0
Primary Antibodies

AB313585

Anti-LOXL1 antibody [EPR28297-2]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-LOXL1 antibody [EPR28297-2] (AB313585)

  • 0
  • 0
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

ab267495 is the carrier-free version of ab260046.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine) (PubMed : 27735137). Acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation (PubMed : 27735137). Shows no activity against histone H3 when it is trimethylated on 'Lys-9' (H3K9me3) or 'Lys-27' (H3K27me3) or when 'Lys-4' is monomethylated (H3K4me1) or dimethylated (H3K4me2) (PubMed : 27735137). Also mediates deamination of methylated TAF10, a member of the transcription factor IID (TFIID) complex, which induces release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription (PubMed : 25959397). LOXL2-mediated deamination of TAF10 results in transcriptional repression of genes required for embryonic stem cell pluripotency including POU5F1/OCT4, NANOG, KLF4 and SOX2 (By similarity). Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin CDH1, probably by mediating deamination of histone H3 (PubMed : 16096638, PubMed : 24414204, PubMed : 27735137). During EMT, involved with SNAI1 in negatively regulating pericentromeric heterochromatin transcription (PubMed : 24239292). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (PubMed : 24239292). Interacts with the endoplasmic reticulum protein HSPA5 which activates the IRE1-XBP1 pathway of the unfolded protein response, leading to expression of several transcription factors involved in EMT and subsequent EMT induction (PubMed : 28332555). Involved in E-cadherin repression following hypoxia, a hallmark of EMT believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression (PubMed : 20026874). When secreted into the extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin (PubMed : 20306300). Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding (PubMed : 21835952). Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation (By similarity).
See full target information LOXL2

대체 명칭 보기

Lysyl oxidase homolog 2, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14, LOXL2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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