AB239585

Anti-LPCAT3 antibody [6-2#]

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(8 제품이 사용된 논문 )

Mouse Monoclonal LPCAT3 antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Pig samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LPCAT3 aa 100-250.

대체 명칭 보기

MBOAT5, OACT5, LPCAT3, Lysophospholipid acyltransferase 5, LPLAT 5, 1-acylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphoethanolamine O-acyltransferase, 1-acylglycerophosphoserine O-acyltransferase, Lysophosphatidylcholine acyltransferase, Lysophosphatidylcholine acyltransferase 3, Lysophosphatidylserine acyltransferase, Membrane-bound O-acyltransferase domain-containing protein 5, LPCAT, Lyso-PC acyltransferase, Lyso-PC acyltransferase 3, LPSAT, Lyso-PS acyltransferase, O-acyltransferase domain-containing protein 5

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT3 antibody [6-2#] (AB239585)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT3 antibody [6-2#] (AB239585)

Paraffin-embedded human glioma tissue stained for LPCAT3 using ab239585 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT3 antibody [6-2#] (AB239585)

Paraffin-embedded human brain tissue stained for LPCAT3 using ab239585 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT3 antibody [6-2#] (AB239585)

Paraffin-embedded human breast cancer tissue stained for LPCAT3 using ab239585 at 30 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT3 antibody [6-2#] (AB239585)

Paraffin-embedded human liver tissue stained for LPCAT3 using ab239585 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Lab

Western blot - Anti-LPCAT3 antibody [6-2#] (AB239585)

Western blot : Anti-LPCAT3 antibody [6-2#] ab239585 staining at 2 µg/mL, shown in green; Rabbit anti-ACTN4 ab108198 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 56 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in LPCAT3 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW and Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-LPCAT3 antibody [6-2#] (ab239585) at 2 µg/mL

Lane 1:

Wild-type MCF7 cell lysates at 20 µg

Lane 2:

LPCAT3 knockout MCF7 cell lysates at 20 µg

Lane 3:

HepG2 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Supplier Data

Western blot - Anti-LPCAT3 antibody [6-2#] (AB239585)

Lanes 1 - 4:

Western blot - Anti-LPCAT3 antibody [6-2#] (ab239585) at 3 µg/mL

Lane 5:

Western blot - Anti-LPCAT3 antibody [6-2#] (ab239585) at 3 µg

Lane 1:

Human serum

Lane 2:

Pig liver lysate

Lane 3:

Human liver lysate

Lane 4:

Pig pancreas lysate

Lane 5:

Pig kidney lysate

Secondary

All lanes:

HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution

Predicted band size: 56 kDa

false

Supplier Data

Western blot - Anti-LPCAT3 antibody [6-2#] (AB239585)

All lanes:

Western blot - Anti-LPCAT3 antibody [6-2#] (ab239585) at 5 µg/mL

All lanes:

Recombinant human LPCAT3 protein

Predicted band size: 56 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

6-2#

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

Reacts with

Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human LPCAT3 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q6P1A2

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Lysophospholipid O-acyltransferase (LPLAT) that catalyzes the reacylation step of the phospholipid remodeling process also known as the Lands cycle (PubMed : 18195019, PubMed : 18772128, PubMed : 18782225). Catalyzes transfer of the fatty acyl chain from fatty acyl-CoA to 1-acyl lysophospholipid to form various classes of phospholipids. Converts 1-acyl lysophosphatidylcholine (LPC) into phosphatidylcholine (PC) (LPCAT activity), 1-acyl lysophosphatidylserine (LPS) into phosphatidylserine (PS) (LPSAT activity) and 1-acyl lysophosphatidylethanolamine (LPE) into phosphatidylethanolamine (PE) (LPEAT activity) (PubMed : 18195019, PubMed : 18772128, PubMed : 18782225). Favors polyunsaturated fatty acyl-CoAs as acyl donors compared to saturated fatty acyl-CoAs (PubMed : 18195019, PubMed : 18772128). Has higher activity for LPC acyl acceptors compared to LPEs and LPSs. Can also transfer the fatty acyl chain from fatty acyl-CoA to 1-O-alkyl lysophospholipid or 1-O-alkenyl lysophospholipid with lower efficiency (By similarity). Acts as a major LPC O-acyltransferase in liver and intestine. As a component of the liver X receptor/NR1H3 or NR1H2 signaling pathway, mainly catalyzes the incorporation of arachidonate into PCs of endoplasmic reticulum (ER) membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Promotes processing of sterol regulatory protein SREBF1 in hepatocytes, likely by facilitating the translocation of SREBF1-SCAP complex from ER to the Golgi apparatus (By similarity). Participates in mechanisms by which the liver X receptor/NR1H3 or NR1H2 signaling pathway counteracts lipid-induced ER stress response and inflammation. Down-regulates hepatic inflammation by limiting arachidonic acid availability for synthesis of inflammatory eicosanoids, such as prostaglandins (By similarity). In enterocytes, acts as a component of a gut-brain feedback loop that coordinates dietary lipid absorption and food intake. Regulates the abundance of PCs containing linoleate and arachidonate in enterocyte membranes, enabling passive diffusion of fatty acids and cholesterol across the membrane for efficient chylomicron assembly (By similarity). In the intestinal crypt, acts as a component of dietary-responsive phospholipid-cholesterol axis, regulating the biosynthesis of cholesterol and its mitogenic effects on intestinal stem cells (By similarity).

See full target information LPCAT3

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제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 16:542 PubMed40691131

2025

HIF-1α alleviates ferroptosis in ulcerative colitis by regulation of GPX4.

Applications

Unspecified application

Species

Unspecified reactive species

Weitao Hu,Yanliang Cai,Daxing Cai,Zongchi Chen,Siying Huang,Su Zhang,Huie Zhuang,Taiyong Fang,Xiaoqing Chen

International journal of biological sciences 21:1275-1293 PubMed39897035

2025

Exosomal mediated lipid metabolism, ferritinophagy and CoQ-dependent pathway contributes to the ferroptosis of keratinocyte in SJS/TEN.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Pei Qiao,ChunYing Xiao,ZiPeng Cao,JiaoLing Chen,Hui Fang,JianKang Yang,ZeHua Kang,ErLe Dang,Shuai Shao,BingYu Pang,QingYang Li,ZhenLai Zhu,ShengXian Shen,Akito Hasegawa,Riichiro Abe,HongJiang Qiao,Gang Wang,Meng Fu

Nature communications 15:7611 PubMed39218970

2024

Suppression of ferroptosis by vitamin A or radical-trapping antioxidants is essential for neuronal development.

Applications

Unspecified application

Species

Unspecified reactive species

Juliane Tschuck,Vidya Padmanabhan Nair,Ana Galhoz,Carole Zaratiegui,Hin-Man Tai,Gabriele Ciceri,Ina Rothenaigner,Jason Tchieu,Brent R Stockwell,Lorenz Studer,Daphne S Cabianca,Michael P Menden,Michelle Vincendeau,Kamyar Hadian

Nature cell biology 26:811-824 PubMed38671262

2024

LPCAT1-mediated membrane phospholipid remodelling promotes ferroptosis evasion and tumour growth.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwen Li,Yameng Hu,Haiqing Zheng,Man Li,Yuanji Liu,Rongni Feng,Xincheng Li,Shuxia Zhang,Miaoling Tang,Meisongzhu Yang,Ruyuan Yu,Yingru Xu,Xinyi Liao,Suwen Chen,Wanying Qian,Qiliang Zhang,Daolin Tang,Bo Li,Libing Song,Jun Li

Methods in molecular biology (Clifton, N.J.) 2712:211-222 PubMed37578709

2023

Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhong,Ruochan Chen

Research (Washington, D.C.) 2022:0006 PubMed39290970

2022

Lipidome Atlas of the Developing Heart Uncovers Dynamic Membrane Lipid Attributes Underlying Cardiac Structural and Metabolic Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Miao,Bowen Li,Zehua Wang,Jinming Mu,Yanlin Tian,Binhua Jiang,Shaohua Zhang,Xia Gong,Guanghou Shui,Sin Man Lam

Pharmaceutical biology 60:2229-2236 PubMed36367996

2022

Ginsenoside Rb1 reduces oxidative/carbonyl stress damage and ameliorates inflammation in the lung of streptozotocin-induced diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Su,Cheng-Ju Tian,Ying Wang,Jiaojiao Shi,Xiaoxiao Chen,Zhong Zhen,Yu Bai,Lan Deng,Chunpeng Feng,Zhuang Ma,Jinfeng Liu

Annals of translational medicine 9:1381 PubMed34733933

2021

Dipetidyl peptidase-4 and transferrin receptor serve as prognostic biomarkers for acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Wei,Guan Ye Nai,Yi Dai,Xun Jun Huang,Ming Yue Xiong,Xiang You Yao,Zhi Ning Huang,Si Nian Li,Wei Jie Zhou,Yan Huang,Peng Cheng,Dong Hong Deng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-LPCAT3 antibody [6-2#]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT3 antibody [6-2#] (AB239585)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT3 antibody [6-2#] (AB239585)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT3 antibody [6-2#] (AB239585)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LPCAT3 antibody [6-2#] (AB239585)

Western blot - Anti-LPCAT3 antibody [6-2#] (AB239585)

Western blot - Anti-LPCAT3 antibody [6-2#] (AB239585)

Western blot - Anti-LPCAT3 antibody [6-2#] (AB239585)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (8)

Product promise