Anti-LRP4 antibody [EPR30692-584]

• WB

Supplier Data

Western blot - Anti-LRP4 antibody [EPR30692-584] (AB324976)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : RAW 264.7, Beta-TC-6, Raji, HDLM-2

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 19936252; PMID : 26847765; PMID : 17119023).

Lanes 1-4 of this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LRP4 antibody [EPR30692-584] (ab324976) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 48 µg

Lane 2:

MEF (mouse embryo fibroblast) whole cell lysate at 48 µg

Lane 3:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 48 µg

Lane 4:

Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 48 µg

Lane 5:

SK-N-BE(2) (human neuroblastoma neuroblast) whole cell lysate at 48 µg

Lane 6:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 48 µg

Lane 7:

NCI-H69 (human lung small cell carcinoma cell line) whole cell lysate at 48 µg

Lane 8:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 48 µg

Lane 9:

HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 270 kDa,124 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-LRP4 antibody [EPR30692-584] (AB324976)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : colon(adult), testis(adult), skeletal muscle(adult).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 19936252; PMID : 26847765; PMID : 17119023).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LRP4 antibody [EPR30692-584] (ab324976) at 1/1000 dilution

Lane 1:

Mouse E18 brain tissue lysate at 20 µg

Lane 2:

Mouse E18 skeletal muscle tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 5:

Mouse colon tissue lysate at 20 µg

Lane 6:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 270 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-LRP4 antibody [EPR30692-584] (AB324976)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : colon(adult), skeletal muscle(adult).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 19936252; PMID : 26847765; PMID : 17119023).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LRP4 antibody [EPR30692-584] (ab324976) at 1/1000 dilution

Lane 1:

Rat E15 brain tissue lysate at 48 µg

Lane 2:

Rat fetal brain tissue lysate at 48 µg

Lane 3:

Rat lung tissue lysate at 48 µg

Lane 4:

Rat skeletal muscle tissue lysate at 48 µg

Lane 5:

Rat colon tissue lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 270 kDa,124 kDa

false

Exposure time: 180s