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AB326674

Anti-LRP6 (phospho S1490) antibody [SR1321]

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Rabbit Recombinant Monoclonal LRP6 phospho S1490 antibody. Suitable for WB and reacts with Human samples.
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Western blot - Anti-LRP6 (phospho S1490) antibody [SR1321] (AB326674)
  • WB

Supplier Data

Western blot - Anti-LRP6 (phospho S1490) antibody [SR1321] (AB326674)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : HeLa cells untreated.
Lane 2 : treated 5 hours with conditioned medium from L cells overexpressing Wnt3a
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size : 180 kDa
Observed band size : 180-210 kDa
Exposure time : 6 seconds

All lanes:

Western blot - Anti-LRP6 (phospho S1490) antibody [SR1321] (ab326674) at 1/1000 dilution

Lane 1:

HeLa cells untreated at 20 µg

Lane 2:

HeLa cells treated 5 hours with conditioned medium from L cells overexpressing Wnt3a at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 180 kDa

Observed band size: 180-210 kDa

false

Exposure time: 6s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1321

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB134146

Anti-LRP6 antibody [EPR2423(2)]

RabMAb|Recombinant|KO Validated

Western blot - Anti-LRP6 antibody [EPR2423(2)] (AB134146)

  • 4
  • 5
Primary Antibodies

AB226758

Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody

Indirect ELISA - Anti-LRP6 (phospho S1490) + LRP5 (phospho S1503) antibody (AB226758)

  • 0
  • 0
Sample Prep & Detection Kits

AB188285

FITC Conjugation Kit (Fast) - Lightning-Link®

Fluorescence Microscopy - FITC Conjugation Kit (Fast) - Lightning-Link® (AB188285)

  • 5
  • 1
Primary Antibodies

AB36121

Anti-LRP5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRP5 antibody (AB36121)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes (PubMed : 11357136, PubMed : 11448771, PubMed : 15778503, PubMed : 16341017, PubMed : 16513652, PubMed : 17326769, PubMed : 17400545, PubMed : 19107203, PubMed : 19293931, PubMed : 19801552, PubMed : 28341812, PubMed : 34896607). Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in various processes including retinal angiogenesis and bone formation (PubMed : 11357136, PubMed : 11448771, PubMed : 15778503, PubMed : 16341017, PubMed : 16513652, PubMed : 17326769, PubMed : 17400545, PubMed : 19107203, PubMed : 19293931, PubMed : 19801552, PubMed : 28341812, PubMed : 34896607). The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalosomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin (PubMed : 16513652). Required for posterior patterning of the epiblast during gastrulation (By similarity).
See full target information LRP6 pS1490

대체 명칭 보기

Low-density lipoprotein receptor-related protein 6, LRP-6, LRP6
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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