AB133488

Anti-LRPAP1 antibody [EPR3328]

RabMAb
Recombinant
KO Validated
각 태그의 뜻

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Rabbit Recombinant Monoclonal LRPAP1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

A2MRAP, LRPAP1, Alpha-2-macroglobulin receptor-associated protein, Alpha-2-MRAP, Low density lipoprotein receptor-related protein-associated protein 1, RAP

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (AB133488)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (AB133488)

Immunohistochemical analysis of LRPAP1 in paraffin embedded Human kidney tissue using ab133488 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (AB133488)

Immunohistochemical analysis of LRPAP1 in paraffin embedded Human brain tissue using ab133488 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-LRPAP1 antibody [EPR3328] (AB133488)

Western blot : Anti-LRPAP1 antibody [EPR3328] ab133488 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 41 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in LRPAP1 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

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Western blot - Anti-LRPAP1 antibody [EPR3328] (ab133488) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG cell lysates at 20 µg

Lane 2:

LRPAP1 knockout U-87 MG cell lysates at 20 µg

Lane 3:

PC-3 cell lysates at 20 µg

Lane 4:

HDLM-2 cell lysates at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

false

Unknown

Western blot - Anti-LRPAP1 antibody [EPR3328] (AB133488)

All lanes:

Western blot - Anti-LRPAP1 antibody [EPR3328] (ab133488) at 1/1000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

BxPC-3 cell lysate at 10 µg

Lane 5:

NCCIT lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 41 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3328

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway.

See full target information LRPAP1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental and therapeutic medicine 28:400 PubMed39171145

2024

GAB1 attenuates lipopolysaccharide‑mediated endothelial dysfunction via regulation of SOCS3.

Applications

Unspecified application

Species

Unspecified reactive species

Guangdong Ren,Ran Liu,Huiqiang Mai,Gang Yin,Fulai Ding,Chunmei Wang,Shuxin Chen,Xianqi Lan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LRPAP1 antibody [EPR3328]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (AB133488)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LRPAP1 antibody [EPR3328] (AB133488)

Western blot - Anti-LRPAP1 antibody [EPR3328] (AB133488)

Western blot - Anti-LRPAP1 antibody [EPR3328] (AB133488)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise